Zidorn C, Stuppner H
Institut für Pharmazie der Universität Innsbruck, Abteilung Für Pharmakognosie, Josef-Moeller-Haus, Innrain 52, A-6020, Innsbruck, Austria
Biochem Syst Ecol. 2001 Aug;29(8):827-837. doi: 10.1016/s0305-1978(01)00019-9.
A chemosystematic study of the subgenus Oporinia of the genus Leontodon (Asteraceae) was performed, using flavonoids and phenolic acids in the flowerheads as diagnostic characters. A total of 44 samples from nine different Oporinia taxa were analyzed. Five luteolin-derivatives (luteolin, luteolin 7-O-beta-D-gentiobioside, luteolin 7-O-beta-D-glucoside, luteolin 7-O-beta-D-glucuronide, and luteolin 4'-O-beta-D-glucoside) and four caffeic acid derivatives (caffeoyl tartaric acid, chlorogenic acid, cichoric acid, and 3,5-dicaffeoylquinic acid) were identified in crude extracts by means of HPLC retention times, on-line UV spectra and on-line MS spectra. Quantification of these compounds was performed by HPLC, using quercetin as internal standard. The data obtained were processed by Principal Component Analysis, resulting in the formation of five different clusters. These clusters were taxonomically interpretable and are in good agreement with the morphologically based system of the genus Leontodon.
以菊科狮牙草属的孔孢亚属为研究对象，开展了一项化学分类学研究，将头状花序中的黄酮类化合物和酚酸作为诊断特征。对来自9个不同孔孢类群的44个样本进行了分析。通过高效液相色谱保留时间、在线紫外光谱和在线质谱，在粗提物中鉴定出5种木犀草素衍生物（木犀草素、木犀草素7 - O - β - D - 龙胆二糖苷、木犀草素7 - O - β - D - 葡萄糖苷、木犀草素7 - O - β - D - 葡萄糖醛酸苷和木犀草素4'- O - β - D - 葡萄糖苷）和4种咖啡酸衍生物（咖啡酰酒石酸、绿原酸、菊苣酸和3,5 - 二咖啡酰奎宁酸）。以槲皮素为内标，通过高效液相色谱对这些化合物进行定量分析。所得数据经主成分分析处理，形成了5个不同的聚类。这些聚类在分类学上具有可解释性，与基于形态学的狮牙草属分类系统高度一致。
