检测甲型流感病毒非结构蛋白（NS1）抗体可区分接种疫苗的马匹和感染病毒的马匹。

Detection of antibodies to the nonstructural protein (NS1) of influenza A virus allows distinction between vaccinated and infected horses.

作者信息

Ozaki H, Sugiura T, Sugita S, Imagawa H, Kida H

机构信息

Department of Disease Control, Hokkaido University Graduate School of Veterinary Medicine, Sapporo 060-0818, Japan.

出版信息

Vet Microbiol. 2001 Sep 20;82(2):111-9. doi: 10.1016/s0378-1135(01)00366-2.

DOI:10.1016/s0378-1135(01)00366-2

PMID:11423202

Abstract

Antibodies to the nonstructural protein (NS1) of A/equine/Miami/1/63 (H3N8) influenza virus were detected exclusively in the sera of mice experimentally infected with A/Aichi/2/68 (H3N2) and horses infected with A/equine/Kentucky/1/81 (H3N8) or A/equine/La Plata/1/93 (H3N8), but not in those of the animals immunized with the inactivated viruses, by enzyme-linked immunosorbent assay (ELISA) using a recombinant NS1 as antigen. The results indicate that the present method is useful for serological diagnosis to distinguish horses infected with equine H3 influenza viruses from those immunized with the inactivated vaccine.

摘要

通过使用重组NS1作为抗原的酶联免疫吸附测定（ELISA），在实验感染A/爱知/2/68（H3N2）的小鼠血清以及感染A/马/肯塔基/1/81（H3N8）或A/马/拉普拉塔/1/93（H3N8）的马血清中，专门检测到了针对A/马/迈阿密/1/63（H3N8）流感病毒非结构蛋白（NS1）的抗体，但在使用灭活病毒免疫的动物血清中未检测到。结果表明，本方法可用于血清学诊断，以区分感染马H3流感病毒的马和接种过灭活疫苗的马。