犬类至少有三种基因不同的小型梨形虫。

There are at least three genetically distinct small piroplasms from dogs.

作者信息

Kjemtrup A M, Kocan A A, Whitworth L, Meinkoth J, Birkenheuer A J, Cummings J, Boudreaux M K, Stockham S L, Irizarry-Rovira A, Conrad P A

机构信息

Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, USA.

出版信息

Int J Parasitol. 2000 Dec;30(14):1501-5. doi: 10.1016/s0020-7519(00)00120-x.

DOI:10.1016/s0020-7519(00)00120-x

PMID:11428342

Abstract

The 18S nuclear subunit ribosomal RNA (18S rRNA) gene of small piroplasms isolated from dogs from Okinawa (Japan), Oklahoma, North Carolina, Indiana, Missouri, and Alabama, was isolated and sequenced. Phylogenetic analysis of these sequences and comparisons with sequences from other Babesia, Cytauxzoon, and Theileria species revealed that all canine small babesial isolates, with the exception of isolates from California and Spain, were placed in a group containing the Babesia spp. sensu stricto. Within the Babesia spp. sensu stricto, there was support for separating the small canine piroplasms from the large canine piroplasm, Babesia canis. The isolate from California was in a distinct phylogenetic clade, closely related to babesial isolates from wildlife and humans from the Western US. The canine isolate from Spain was closely related to Babesia microti. These results suggest that there are multiple small piroplasm species in dogs. The isolates from the Midwestern and Eastern US and the one from Japan probably represent a single species with wide geographic distribution.

摘要

从日本冲绳、俄克拉荷马州、北卡罗来纳州、印第安纳州、密苏里州和阿拉巴马州的犬类中分离出小型梨形虫的18S核糖体核糖核酸（18S rRNA）基因并进行测序。对这些序列进行系统发育分析，并与其他巴贝斯虫属、嗜吞噬细胞无形体属和泰勒虫属的序列进行比较，结果显示，除了来自加利福尼亚州和西班牙的分离株外，所有犬类小型巴贝斯虫分离株都被归入一个包含狭义巴贝斯虫属的类群。在狭义巴贝斯虫属内，有证据支持将小型犬梨形虫与大型犬梨形虫——犬巴贝斯虫区分开来。来自加利福尼亚州的分离株处于一个独特的系统发育分支中，与来自美国西部野生动物和人类的巴贝斯虫分离株密切相关。来自西班牙的犬类分离株与微小巴贝斯虫密切相关。这些结果表明犬类中存在多种小型梨形虫物种。来自美国中西部和东部的分离株以及来自日本的分离株可能代表了一个具有广泛地理分布的单一物种。