Gosiewska A, Rezania A, Dhanaraj S, Vyakarnam M, Zhou J, Burtis D, Brown L, Kong W, Zimmerman M, Geesin J C
Johnson & Johnson Wound Healing Technology Resource Center, Skillman, New Jersey 08858, USA.
Tissue Eng. 2001 Jun;7(3):267-77. doi: 10.1089/10763270152044134.
The ability of synthetic or natural scaffolds to support invasion of cells from surrounding tissue is a key parameter for tissue engineering (TE). In this study, the migration of fibroblasts, chondrocytes, and osteoblasts into biodegradable polymer scaffolds was evaluated using a novel, three-dimensional (3-D) transmigration assay. This assay is based on a cell-populated contracted collagen lattice with a biodegradable polymer scaffold implanted at the center of the collagen gel. Cell migration into the scaffolds was assessed both quantitatively and qualitatively following various time lengths in culture using image analysis. Chondrocytes, incorporated within the collagen lattice, migrated into polymer scaffolds, when cultured both statically or in a rotating bioreactor. However, the bioreactor cultures resulted in a significantly greater cell invasion as compared to static cultures. There was a cell density-dependent osteoblast migration from collagen lattice into polymer scaffold, when tested in the transmigration assay. In addition, polymer scaffolds, treated with or without recombinant human platelet-derived growth factor (rh-PDGF-BB) were evaluated for fibroblast migration. The presence of rh-PDGF-BB resulted in significantly greater fibroblast invasion as compared to untreated scaffolds. Our studies suggest that the transmigration model provides a rapid system for testing cell invasion of potential scaffolds for tissue engineering applications.
合成或天然支架支持周围组织细胞侵袭的能力是组织工程（TE）的一个关键参数。在本研究中，使用一种新型的三维（3-D）迁移试验评估了成纤维细胞、软骨细胞和成骨细胞向可生物降解聚合物支架的迁移。该试验基于一个细胞填充的收缩胶原晶格，在胶原凝胶中心植入一个可生物降解聚合物支架。使用图像分析在培养不同时间长度后对细胞向支架的迁移进行了定量和定性评估。当在静态或旋转生物反应器中培养时，包含在胶原晶格中的软骨细胞迁移到聚合物支架中。然而，与静态培养相比，生物反应器培养导致细胞侵袭显著增加。在迁移试验中测试时，成骨细胞从胶原晶格向聚合物支架的迁移存在细胞密度依赖性。此外，评估了用或不用重组人血小板衍生生长因子（rh-PDGF-BB）处理的聚合物支架对成纤维细胞迁移的影响。与未处理的支架相比，rh-PDGF-BB的存在导致成纤维细胞侵袭显著增加。我们的研究表明，迁移模型为测试用于组织工程应用的潜在支架的细胞侵袭提供了一个快速系统。
