Dunzel A, Rüdiger T, Pretzel D, Kopsch V, Endres M, Kaps C, Föhr P, Burgkart R H, Linß S, Kinne R W
AG Experimentelle Rheumatologie, Lehrstuhl für Orthopädie, Universitätsklinikum Jena, Klosterlausnitzer Str. 81, 07607 Eisenberg, Deutschland.
Orthopade. 2013 Apr;42(4):254-61. doi: 10.1007/s00132-012-1954-3.
The limited regeneration capacity of hyaline articular cartilage requires detailed studies concerning the tissue integration of cartilage transplants with meaningful but time and/or resource-consuming and in part ethically problematic animal models or, alternatively, with in vitro test systems for implant materials.
The present study describes a regeneration model with bovine cartilage rings (outer Ø 6 mm, central defect Ø 2 mm) for insertion, cultivation and biomechanical or histological testing of cartilage replacement materials (HE and safranin O staining). In this study, resorbable polymers composed of polyglycolic acid (PGA) were analyzed.
Biomechanical testing showed a continuous decrease of the push-out force for the PGA inserts from the cartilage rings, probably due to the resorbability of the material. Histologically, clear immigration of cells into cell-free PGA was observed even after 4 weeks of culture, but in particular after 10 weeks. In addition, storage of proteoglycans was interpreted as an initial sign of the formation of new matrix.
Thus, the new regeneration model is in principle suitable for the testing of biomaterials, but shows limitations in assessing the "lateral bonding" of resorbable materials.
透明关节软骨的再生能力有限，这需要针对软骨移植组织整合进行详细研究，这涉及有意义但耗时和/或耗费资源且部分存在伦理问题的动物模型，或者采用植入材料的体外测试系统。
本研究描述了一种用于软骨替代材料（苏木精-伊红染色和番红O染色）的插入、培养以及生物力学或组织学测试的牛软骨环（外径6毫米，中心缺损直径2毫米）再生模型。在本研究中，对由聚乙醇酸（PGA）组成的可吸收聚合物进行了分析。
生物力学测试表明，PGA植入物从软骨环中的推出力持续下降，这可能是由于材料的可吸收性。组织学上，即使在培养4周后，尤其是10周后，也观察到细胞明显迁移至无细胞的PGA中。此外，蛋白聚糖的储存被解释为新基质形成的初步迹象。
因此，新的再生模型原则上适用于生物材料的测试，但在评估可吸收材料的“侧向结合”方面存在局限性。
