Gardner H W, Grove M J
Mycotoxin Research, NCAUR, ARS, USDA, Peoria, Illinois 61604, USA.
Lipids. 2001 May;36(5):529-33. doi: 10.1007/s11745-001-0753-1.
Seed from maize (corn) Zea mays provides a ready source of 9-lipoxygenase that oxidizes linoleic acid and linolenic acid into 9(S)-hydroperoxy-10(E),12(Z)-octadecadienoic acid and 9(S)-hydroperoxy-10(E),12(Z),15(Z)-octadecatrienoic acid, respectively. Corn seed has a very active hydroperoxide-decomposing enzyme, allene oxide synthase (AOS), which must be removed prior to oxidizing the fatty acid. A simple pH 4.5 treatment followed by centrifugation removes most of the AOS activity. Subsequent purification by ammonium sulfate fractional precipitation results in negligible improvement in 9-hydroperoxide formation. This facile alternative method of preparing 9-hydroperoxides has advantages over other commonly used plant lipoxygenases.
玉米（Zea mays）种子是9-脂氧合酶的现成来源，该酶可将亚油酸和亚麻酸分别氧化为9(S)-氢过氧-10(E),12(Z)-十八碳二烯酸和9(S)-氢过氧-10(E),12(Z),15(Z)-十八碳三烯酸。玉米种子具有一种非常活跃的氢过氧化物分解酶——丙二烯氧化物合酶（AOS），在氧化脂肪酸之前必须将其去除。简单的pH 4.5处理后再进行离心，可去除大部分AOS活性。随后通过硫酸铵分级沉淀进行纯化，9-氢过氧化物的生成量几乎没有改善。这种制备9-氢过氧化物的简便替代方法比其他常用的植物脂氧合酶具有优势。
