Lei L, Altstaedt J, von der Ohe M, Proft T, Gross U, Rink L
Institute of Immunology and Transfusion Medicine, University of Lübeck School of Medicine, D-23538 Lübeck, Germany.
J Leukoc Biol. 2001 Jul;70(1):80-6.
Neutrophils have been shown to express major histocompatibility complex class II (MHC II) after stimulation. However, reports concerning the functional effect of MCH II expression are still lacking. In our hands, granulocyte-monocyte colony-stimulating factor (GM-CSF) alone and in combination with interferon (IFN)-gamma, but not IFN-gamma or interleukin (IL)-3, induced a significant level of expression of human leukocyte antigen DR on neutrophils. The addition of staphylococcal enterotoxin E to neutrophils resulted in a significant increase in IL-8 production only after prestimulation with GM-CSF alone or in combination with IFN-gamma but had no effect on neutrophils preincubated with IFN-gamma alone or IL-3. Staphylococcal enterotoxin A, another bivalent superantigen, also stimulated production of IL-8 by preincubated polymorphonuclear neutrophils, whereas staphylococcal enterotoxin A mutants that are not able to cross-link MHC II molecules failed to induce IL-8 production. Taken together, our results clearly demonstrate that after induction of MHC II, neutrophils are able to respond to MHC II-specific stimulation. These findings support the ideas that the induced MHC II complex is completely functional and that neutrophils may be able to present antigens.
已证实中性粒细胞在受到刺激后可表达主要组织相容性复合体II类分子（MHC II）。然而，关于MHC II表达的功能效应的报道仍然缺乏。在我们的研究中，单独的粒细胞-巨噬细胞集落刺激因子（GM-CSF）以及与干扰素（IFN）-γ联合使用，但不是IFN-γ或白细胞介素（IL）-3，可诱导中性粒细胞上人类白细胞抗原DR的显著表达。向中性粒细胞中添加葡萄球菌肠毒素E，仅在单独或与IFN-γ联合使用GM-CSF进行预刺激后，IL-8的产生才会显著增加，但对单独用IFN-γ或IL-3预孵育的中性粒细胞没有影响。另一种双价超抗原葡萄球菌肠毒素A，也能刺激预先孵育的多形核中性粒细胞产生IL-8，而不能交联MHC II分子的葡萄球菌肠毒素A突变体则不能诱导IL-8的产生。综上所述，我们的结果清楚地表明，在诱导MHC II后，中性粒细胞能够对MHC II特异性刺激作出反应。这些发现支持了以下观点：诱导的MHC II复合体具有完全功能，并且中性粒细胞可能能够呈递抗原。
