McDougal V V, Guarino L A
Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843-2128, USA.
J Virol. 2001 Aug;75(15):7206-9. doi: 10.1128/JVI.75.15.7206-7209.2001.
P143 is a DNA helicase that tightly binds both double-stranded and single-stranded DNA. DNA-protein complexes rapidly dissociated in the presence of ATP and Mg(2+). This finding suggests that ATP hydrolysis causes a conformational change in P143 which decreases affinity for DNA. This supports the model of an inchworm mechanism of DNA unwinding.
P143是一种DNA解旋酶,它能紧密结合双链和单链DNA。在ATP和Mg(2+)存在的情况下,DNA - 蛋白质复合物会迅速解离。这一发现表明,ATP水解会导致P143发生构象变化,从而降低其对DNA的亲和力。这支持了DNA解旋的尺蠖机制模型。