苜蓿银纹夜蛾核型多角体病毒p143基因编码一种DNA解旋酶。
The Autographa californica nuclear polyhedrosis virus p143 gene encodes a DNA helicase.
作者信息
McDougal V V, Guarino L A
机构信息
Departments of Biochemistry & Biophysics, Texas A&M University, College Station, Texas 77843-2128, USA.
出版信息
J Virol. 2000 Jun;74(11):5273-9. doi: 10.1128/jvi.74.11.5273-5279.2000.
Abstract
The P143 protein of Autographa californica nuclear polyhedrosis virus is essential for replication of viral DNA. To determine the function of P143, the protein was purified to near homogeneity from recombinant baculovirus-infected cells that overexpress P143. ATPase activity copurified with P143 protein during purification and also during gel filtration at a high salt concentration. The ATPase activity did not require the presence of single-stranded DNA, but was stimulated fourfold by the addition of single-stranded DNA. The ATPase activity of P143 had a K(m) of 60 microM and a turnover of 4.5 molecules of ATP hydrolyzed/s/molecule of enzyme, indicating moderate affinity for ATP and high catalytic efficiency. P143 unwound a 40-nucleotide primer in an ATP-dependent manner, indicating that the enzyme possesses in vitro DNA helicase activity. Based on this result, it seems likely that P143 functions as a helicase in viral DNA replication.
摘要
苜蓿银纹夜蛾核型多角体病毒的P143蛋白对病毒DNA的复制至关重要。为了确定P143的功能，该蛋白从过表达P143的重组杆状病毒感染细胞中纯化至接近均一状态。在纯化过程以及高盐浓度下的凝胶过滤过程中，ATP酶活性与P143蛋白共纯化。ATP酶活性不需要单链DNA的存在，但添加单链DNA可使其活性提高四倍。P143的ATP酶活性的K(m)为60微摩尔，周转数为每秒每分子酶水解4.5个ATP分子，表明对ATP具有中等亲和力和高催化效率。P143以ATP依赖的方式解开一个40个核苷酸的引物，表明该酶具有体外DNA解旋酶活性。基于这一结果，P143在病毒DNA复制中似乎很可能作为解旋酶发挥作用。
相似文献
1
The Autographa californica nuclear polyhedrosis virus p143 gene encodes a DNA helicase.苜蓿银纹夜蛾核型多角体病毒p143基因编码一种DNA解旋酶。
J Virol. 2000 Jun;74(11):5273-9. doi: 10.1128/jvi.74.11.5273-5279.2000.
2
Autographa californica nuclear polyhedrosis virus p143 gene product is a DNA-binding protein.苜蓿银纹夜蛾核型多角体病毒p143基因产物是一种DNA结合蛋白。
Virology. 1997 Feb 3;228(1):98-106. doi: 10.1006/viro.1996.8361.
3
Characterization of the interaction between P143 and LEF-3 from two different baculovirus species: Choristoneura fumiferana nucleopolyhedrovirus LEF-3 can complement Autographa californica nucleopolyhedrovirus LEF-3 in supporting DNA replication.两种不同杆状病毒物种中P143与LEF-3相互作用的表征：云杉色卷蛾核多角体病毒的LEF-3在支持DNA复制方面可互补苜蓿银纹夜蛾核多角体病毒的LEF-3。
J Virol. 2004 Jan;78(1):329-39. doi: 10.1128/jvi.78.1.329-339.2004.
4
A baculovirus single-stranded DNA binding protein, LEF-3, mediates the nuclear localization of the putative helicase P143.杆状病毒单链DNA结合蛋白LEF-3介导假定解旋酶P143的核定位。
Virology. 1998 Jul 20;247(1):32-40. doi: 10.1006/viro.1998.9235.
5
Extension of Autographa californica nuclear polyhedrosis virus host range by interspecific replacement of a short DNA sequence in the p143 helicase gene.通过种间替换苜蓿银纹夜蛾核型多角体病毒p143解旋酶基因中的一段短DNA序列来扩展其宿主范围
Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):48-52. doi: 10.1073/pnas.91.1.48.
6
The Trichoplusia ni granulovirus helicase is unable to support replication of Autographa californica multicapsid nucleopolyhedrovirus in cells and larvae of T. ni.粉纹夜蛾颗粒体病毒解旋酶无法支持苜蓿银纹夜蛾多粒包埋核型多角体病毒在粉纹夜蛾细胞和幼虫中进行复制。
J Gen Virol. 2000 Jun;81(Pt 6):1593-9. doi: 10.1099/0022-1317-81-6-1593.
7
Baculovirus proteins IE-1, LEF-3, and P143 interact with DNA in vivo: a formaldehyde cross-linking study.杆状病毒蛋白IE-1、LEF-3和P143在体内与DNA相互作用：一项甲醛交联研究。
Virology. 2004 Nov 24;329(2):337-47. doi: 10.1016/j.virol.2004.08.035.
8
DNA and ATP binding activities of the baculovirus DNA helicase P143.杆状病毒DNA解旋酶P143的DNA与ATP结合活性
J Virol. 2001 Aug;75(15):7206-9. doi: 10.1128/JVI.75.15.7206-7209.2001.
9
Site-directed mutagenesis of the AcMNPV p143 gene: effects on baculovirus DNA replication.苜蓿银纹夜蛾核型多角体病毒p143基因的定点诱变：对杆状病毒DNA复制的影响
Virology. 1999 Jan 5;253(1):125-36. doi: 10.1006/viro.1998.9485.
10
Characterization of a putative Spodoptera exigua multicapsid nucleopolyhedrovirus helicase gene.一种推定的甜菜夜蛾多粒包埋核型多角体病毒解旋酶基因的特性分析
J Gen Virol. 1997 Dec;78 ( Pt 12):3101-14. doi: 10.1099/0022-1317-78-12-3101.
引用本文的文献
1
Host AAA+ ATPase TER94 Plays Critical Roles in Building the Baculovirus Viral Replication Factory and Virion Morphogenesis.宿主 AAA+ ATPase TER94 在构建杆状病毒病毒复制工厂和病毒粒子形态发生中发挥关键作用。
J Virol. 2020 Feb 28;94(6). doi: 10.1128/JVI.01674-19.
2
Genomic sequencing of Troides aeacus nucleopolyhedrovirus (TraeNPV) from golden birdwing larvae (Troides aeacus formosanus) to reveal defective Autographa californica NPV genomic features.从金裳凤蝶幼虫（台湾金裳凤蝶）中对 Troides aeacus 核多角体病毒（TraeNPV）进行基因组测序，以揭示缺陷的 Autographa californica NPV 基因组特征。
BMC Genomics. 2019 May 27;20(1):419. doi: 10.1186/s12864-019-5713-2.
3
Autographa californica Nucleopolyhedrovirus AC141 (Exon0), a Potential E3 Ubiquitin Ligase, Interacts with Viral Ubiquitin and AC66 To Facilitate Nucleocapsid Egress.苜蓿银纹夜蛾核型多角体病毒AC141（外显子0），一种潜在的E3泛素连接酶，与病毒泛素和AC66相互作用以促进核衣壳出芽。
J Virol. 2018 Jan 17;92(3). doi: 10.1128/JVI.01713-17. Print 2018 Feb 1.
4
Functional Regulation of an Autographa californica Nucleopolyhedrovirus-Encoded MicroRNA, AcMNPV-miR-1, in Baculovirus Replication.苜蓿银纹夜蛾核型多角体病毒编码的微小RNA（AcMNPV-miR-1）在杆状病毒复制中的功能调控
J Virol. 2016 Jun 24;90(14):6526-6537. doi: 10.1128/JVI.00165-16. Print 2016 Jul 15.
5
Genomic sequencing and analysis of Sucra jujuba nucleopolyhedrovirus.枣尺蠖核型多角体病毒的基因组测序与分析
PLoS One. 2014 Oct 17;9(10):e110023. doi: 10.1371/journal.pone.0110023. eCollection 2014.
6
piggyBac transposon-derived targeting shRNA interference against the Bombyx mori nucleopolyhedrovirus (BmNPV).源自piggyBac转座子的靶向短发夹RNA干扰家蚕核型多角体病毒（BmNPV）
Mol Biol Rep. 2014 Dec;41(12):8247-54. doi: 10.1007/s11033-014-3726-0. Epub 2014 Sep 9.
7
Identification of a high-efficiency baculovirus DNA replication origin that functions in insect and mammalian cells.鉴定一种在昆虫和哺乳动物细胞中起作用的高效杆状病毒DNA复制起点。
J Virol. 2014 Nov;88(22):13073-85. doi: 10.1128/JVI.01713-14. Epub 2014 Sep 3.
8
Screening of candidate proteins interacting with IE-2 of Bombyx mori nucleopolyhedrovirus.筛选与家蚕核型多角体病毒 IE-2 相互作用的候选蛋白。
Mol Biol Rep. 2013 Oct;40(10):5797-804. doi: 10.1007/s11033-013-2683-3.
9
Analysis of the autographa californica multiple nucleopolyhedrovirus overlapping gene pair lef3 and ac68 reveals that AC68 is a per os infectivity factor and that LEF3 is critical, but not essential, for virus replication.分析表明，美洲棉铃虫多核多角体病毒重叠基因对 lef3 和 ac68 中，ac68 是一种经口感染因子，而 lef3 对于病毒复制是关键的，但不是必需的。
J Virol. 2012 Apr;86(7):3985-94. doi: 10.1128/JVI.06849-11. Epub 2012 Jan 25.
10
Baculovirus: molecular insights on their diversity and conservation.杆状病毒：关于其多样性和保守性的分子见解
Int J Evol Biol. 2011;2011:379424. doi: 10.4061/2011/379424. Epub 2011 Apr 11.
本文引用的文献
1
The linker region between the helicase and primase domains of the bacteriophage T7 gene 4 protein is critical for hexamer formation.噬菌体T7基因4蛋白的解旋酶结构域和引发酶结构域之间的连接区域对于六聚体的形成至关重要。
J Biol Chem. 1999 Oct 15;274(42):30303-9. doi: 10.1074/jbc.274.42.30303.
2
Purification of Autographa californica nucleopolyhedrovirus DNA polymerase from infected insect cells.从受感染昆虫细胞中纯化苜蓿银纹夜蛾核多角体病毒DNA聚合酶
J Gen Virol. 1999 Sep;80 ( Pt 9):2519-2526. doi: 10.1099/0022-1317-80-9-2519.
3
Autographa californica nuclear polyhedrosis virus DNA polymerase: measurements of processivity and strand displacement.苜蓿银纹夜蛾核型多角体病毒DNA聚合酶：持续合成能力和链置换的测定
J Virol. 1999 Jun;73(6):4908-18. doi: 10.1128/JVI.73.6.4908-4918.1999.
4
Characterization of the interaction between the baculovirus ssDNA-binding protein (LEF-3) and putative helicase (P143).杆状病毒单链DNA结合蛋白（LEF-3）与假定解旋酶（P143）之间相互作用的特性分析
J Gen Virol. 1999 Feb;80 ( Pt 2):493-500. doi: 10.1099/0022-1317-80-2-493.
5
DnaB from Thermus aquaticus unwinds forked duplex DNA with an asymmetric tail length dependence.嗜热水生栖热菌的DnaB解开具有不对称尾巴长度依赖性的叉状双链DNA。
J Biol Chem. 1999 Mar 12;274(11):6889-97. doi: 10.1074/jbc.274.11.6889.
6
Site-directed mutagenesis of the AcMNPV p143 gene: effects on baculovirus DNA replication.苜蓿银纹夜蛾核型多角体病毒p143基因的定点诱变：对杆状病毒DNA复制的影响
Virology. 1999 Jan 5;253(1):125-36. doi: 10.1006/viro.1998.9485.
7
Biochemical characterization of adeno-associated virus rep68 DNA helicase and ATPase activities.腺相关病毒rep68 DNA解旋酶和ATP酶活性的生化特性
J Virol. 1999 Feb;73(2):1580-90. doi: 10.1128/JVI.73.2.1580-1590.1999.
8
A highly conserved genomic region in baculoviruses: sequence analysis of an 11.3 kbp DNA fragment (46.5-55.1 m.u.) of the Spodoptera exigua multicapsid nucleopolyhedrovirus.杆状病毒中一个高度保守的基因组区域：甜菜夜蛾多粒包埋核多角体病毒11.3 kbp DNA片段（46.5 - 55.1 m.u.）的序列分析
Virus Res. 1998 Jun;55(2):187-98. doi: 10.1016/s0168-1702(98)00040-9.
9
A baculovirus single-stranded DNA binding protein, LEF-3, mediates the nuclear localization of the putative helicase P143.杆状病毒单链DNA结合蛋白LEF-3介导假定解旋酶P143的核定位。
Virology. 1998 Jul 20;247(1):32-40. doi: 10.1006/viro.1998.9235.
10
A DNA helicase from Schizosaccharomyces pombe stimulated by single-stranded DNA-binding protein at low ATP concentration.来自粟酒裂殖酵母的一种DNA解旋酶，在低ATP浓度下受单链DNA结合蛋白刺激。
J Biol Chem. 1997 Jul 25;272(30):18910-9. doi: 10.1074/jbc.272.30.18910.
Zotero 插件