Finkelstein M B, Auringer M P, Halper L A, Linn T C, Singh M, Srere P A
Eur J Biochem. 1979 Aug 15;99(1):209-16. doi: 10.1111/j.1432-1033.1979.tb13247.x.
The polysome fractions involved in the synthesis of the rat-liver inducible lipogenic enzymes, ATP citrate lyase and fatty acid synthetase, were identified by their binding of radioiodinated specific antibodies to enzyme. Both of these populations of specific polysomes were shown to be markedly heavier than specific polysomes involved in albumin synthesis. The quanity of antibody bound to the lipogenic enzyme-related polysomes was markedly affected by the dietary status of the animal. A dietary regimen which induced ipogenesis resulted in a tenfold increase in the hepatic activities of these enzymes found in normally fed animals. The radioactivity bound to hepatic polysomes of induced rats was likewise greater than tenfole higher, presumably reflecting an increase in the number of polysomes active in enzyme synthesis. The fasting state resulted in lower hepatic enzyme activity than normal and correspondingly less binding of ATP citrate lyase and fatty acid synthetase antibodies to the heavy polysomes of the sucrose gradient.
通过放射性碘化的酶特异性抗体与大鼠肝脏诱导性生脂酶、ATP柠檬酸裂解酶和脂肪酸合成酶的结合，鉴定了参与这些酶合成的多核糖体组分。这两类特异性多核糖体均显示比参与白蛋白合成的特异性多核糖体重得多。与生脂酶相关多核糖体结合的抗体量受动物饮食状态的显著影响。诱导脂肪生成的饮食方案导致正常喂养动物肝脏中这些酶的活性增加了10倍。诱导大鼠肝脏多核糖体结合的放射性同样比正常高10倍以上，这可能反映了活跃于酶合成的多核糖体数量增加。禁食状态导致肝脏酶活性低于正常水平，相应地，ATP柠檬酸裂解酶和脂肪酸合成酶抗体与蔗糖梯度重多核糖体的结合也减少。
