大鼠乳腺中脂肪生成酶活性的启动。
Initiation of lipogenic enzyme activities in rat mammary glands.
作者信息
Martyn P, Hansen I A
出版信息
Biochem J. 1981 Jul 15;198(1):187-92. doi: 10.1042/bj1980187.
The activities of acetyl-CoA carboxylase, ATP citrate-lyase and fatty acid synthetase remained low until parturition at 22 days of gestation and increased significantly within 1 day post partum. Administration of progesterone on days 20 and 21 and at parturition abolished the increases for at least 48 h after parturition. Removal of the pups of normal rats prevented the increases in activities of acetyl-CoA carboxylase and ATP citrate-lyase, but not of fatty acid synthetase, and administration of prolactin corticosterone or insulin did not stimulate activity. Tissue from suckled glands in which the ducts had been ligated at parturition showed no increase in the activities of acetyl-CoA carboxylase and ATP citrate-lyase within 24 h, whereas fatty acid synthetase activity was similar to that in the sham-operated contralateral glands. Foetoplacentectomy on day 18 increased the activity of fatty acid synthetase but not of acetyl-CoA carboxylase and ATP citrate-lyase; suckling of these dams by foster pups increased both acetyl-CoA carboxylase and ATP citrate-lyase.
在妊娠22天分娩前，乙酰辅酶A羧化酶、ATP柠檬酸裂解酶和脂肪酸合成酶的活性一直较低，产后1天内显著增加。在妊娠第20天和第21天以及分娩时给予孕酮，可使产后至少48小时内这些酶活性的增加消失。正常大鼠幼崽被移除后，乙酰辅酶A羧化酶和ATP柠檬酸裂解酶的活性增加受到抑制，但脂肪酸合成酶不受影响，且给予催乳素、皮质酮或胰岛素不会刺激其活性。分娩时结扎导管的哺乳乳腺组织在24小时内乙酰辅酶A羧化酶和ATP柠檬酸裂解酶的活性没有增加，而脂肪酸合成酶的活性与假手术对侧乳腺组织相似。妊娠第18天进行胎儿胎盘切除术可增加脂肪酸合成酶的活性，但不增加乙酰辅酶A羧化酶和ATP柠檬酸裂解酶的活性；由代乳幼崽哺乳这些母鼠可使乙酰辅酶A羧化酶和ATP柠檬酸裂解酶的活性均增加。
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