Park J P, Kim S W, Hwang H J, Yun J W
Department of Biotechnology, Taegu University, Kyungsan, Kyungbuk, Korea.
Lett Appl Microbiol. 2001 Jul;33(1):76-81. doi: 10.1046/j.1472-765x.2001.00950.x.
The objective of the present study was to determine the optimal culture conditions for exo-biopolymer production by Cordyceps militaris in shake flask culture.
The optimal temperature and initial pH for both mycelial growth and exo-biopolymer production by Cordyceps militaris in shake flask culture were found to be 20 degrees C and 6.0, respectively. Sucrose (40 g x l(-1)) and corn steep powder (10 g x l(-1)) were the most suitable carbon and nitrogen source for both mycelial growth and exo-biopolymer production.
Under optimal culture conditions, the maximum exo-biopolymer concentration in a 5-l jar fermenter indicated 10.3 g x l(-1), which was approximately three times higher than that in shake flask culture.
This process can have a significant impact on the industrial scale when sucrose and corn steep powder were used as carbon and nitrogen source.
本研究的目的是确定在摇瓶培养中蛹虫草产生胞外生物聚合物的最佳培养条件。
发现在摇瓶培养中，蛹虫草菌丝体生长和胞外生物聚合物产生的最佳温度和初始pH分别为20℃和6.0。蔗糖（40 g·L⁻¹）和玉米浆干粉（10 g·L⁻¹）是菌丝体生长和胞外生物聚合物产生最合适的碳源和氮源。
在最佳培养条件下，5 L罐式发酵罐中胞外生物聚合物的最大浓度为10.3 g·L⁻¹，约为摇瓶培养中浓度的三倍。
当使用蔗糖和玉米浆干粉作为碳源和氮源时，该工艺对工业规模可能有重大影响。
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