Yip Y K, Ramachandran S, Wagle S R
Proc Soc Exp Biol Med. 1975 Jul;149(3):683-7. doi: 10.3181/00379727-149-38879.
The properties of the 500-fold purified high-molecular-weight lipase have been studied. The rate of hydrolysis of the triglycerides decreases with increasing fatty acid chain length. The lipolytic activity also increases with increase in unsaturation in the fatty acyl moiety. Diglycerides are hydrolyzed at more than twice the rate for triglycerides while monoglycerides are not hydrolyzed. Methyl esters are generally hydrolyzed at a higher rate which increases with increasing chain length of the fatty acid but the enzyme does not act on phospholipids. Emulsifying agents such as Tween 20, gum arabic, and albumin increase the rate of hydrolysis. Metal ions such as Hg2+, Zn2+, Cu2+, and Fe2+ strongly inhibit the lipolytic activity of the high-molecular-weight lipase while Ca2+ or Mg2+ by themselves show no stimulating effect. Treatment of the high-molecular-weight lipase with P-chloromercurybenzoate inhibits hydrolytic activity by 70% while iodoacetic acid has no effect.
对500倍纯化的高分子量脂肪酶的性质进行了研究。甘油三酯的水解速率随脂肪酸链长度的增加而降低。脂肪分解活性也随着脂肪酰基部分不饱和度的增加而增加。甘油二酯的水解速率是甘油三酯的两倍多，而甘油单酯不被水解。甲酯通常以较高的速率水解，该速率随脂肪酸链长度的增加而增加，但该酶对磷脂无作用。吐温20、阿拉伯胶和白蛋白等乳化剂可提高水解速率。Hg2+、Zn2+、Cu2+和Fe2+等金属离子强烈抑制高分子量脂肪酶的脂肪分解活性，而Ca2+或Mg2+本身无刺激作用。用对氯汞苯甲酸处理高分子量脂肪酶可使水解活性降低70%，而碘乙酸则无作用。
