Lykidis A, Mougios V, Arzoglou P
Department of Chemistry, Aristotle University of Thessaloniki, Greece.
Clin Chem. 1994 Nov;40(11 Pt 1):2053-6.
Human pancreatic lipase assays are usually performed in the presence of either emulsified triglycerides or diglycerides within the limits of their solubility. Two reactions are catalyzed in the presence of triglycerides: hydrolysis of triglycerides to diglycerides, and diglycerides to monoglycerides. The contribution of each reaction to the final result was determined after extensive kinetic studies on the appearance and/(or) accumulation of intermediates and/(or) products. Acylated glycerides were analyzed after extraction from the reaction mixture, separation of lipid classes by thin-layer chromatography, and quantification by capillary gas chromatography. The results show that after 10 min of reaction in the presence of high concentrations of triolein, 75% of the released fatty acids arise from the first reaction. Relative merits and disadvantages of each substrate (triglyceride or diglyceride) are discussed in terms of practicability.
人胰脂肪酶测定通常在乳化甘油三酯或甘油二酯存在的情况下进行，且在其溶解度范围内。在甘油三酯存在下会催化两个反应：甘油三酯水解为甘油二酯，以及甘油二酯水解为甘油单酯。在对中间体和/或产物的出现和/或积累进行广泛的动力学研究后，确定了每个反应对最终结果的贡献。从反应混合物中提取后，通过薄层色谱法分离脂质类别，并通过毛细管气相色谱法定量分析酰化甘油酯。结果表明，在高浓度三油精存在下反应10分钟后，释放的脂肪酸中有75%来自第一个反应。从实用性的角度讨论了每种底物（甘油三酯或甘油二酯）的相对优缺点。
