Hedlund B P, Geiselbrecht A D, Staley J T
Department of Microbiology, University of Washington, Box 357242, Seattle, WA 98195-7242, USA.
FEMS Microbiol Lett. 2001 Jul 10;201(1):47-51. doi: 10.1111/j.1574-6968.2001.tb10731.x.
One strain of bacteria, designated NCE312, was isolated from a naphthalene-digesting chemostat culture that was inoculated with creosote-contaminated marine sediment. The strain was isolated based on its ability to grow using naphthalene as a sole carbon source. In addition, the strain degraded 2-methylnaphthalene and 1-methylnaphthalene. Analysis of a 16S rRNA gene sequence from NCE312 placed the isolate in the genus Marinobacter. Degenerate PCR primers were used to amplify a fragment of a naphthalene 1,2-dioxygenase large subunit gene. A phylogenetic analysis indicated the Marinobacter naphthalene dioxygenase is similar to those from Pseudomonas and Burkholderia strains suggesting that the dioxygenase gene may have been transferred horizontally between these lineages of bacteria.
从一个用杂酚油污染的海洋沉积物接种的萘消化恒化器培养物中分离出一种名为NCE312的细菌菌株。该菌株是基于其以萘作为唯一碳源生长的能力而分离出来的。此外,该菌株还能降解2-甲基萘和1-甲基萘。对NCE312的16S rRNA基因序列进行分析,将该分离株归入海杆菌属。使用简并PCR引物扩增萘1,2-双加氧酶大亚基基因的一个片段。系统发育分析表明,海杆菌萘双加氧酶与假单胞菌属和伯克霍尔德菌属菌株的双加氧酶相似,这表明双加氧酶基因可能在这些细菌谱系之间水平转移。