视锥细胞反应对大鼠视网膜电图的贡献。

The contribution of cone responses to rat electroretinograms.

作者信息

Nixon P J, Bui B V, Armitage J A, Vingrys A J

机构信息

Department of Optometry and Vision Sciences, University of Melbourne, Victoria, Australia.

出版信息

Clin Exp Ophthalmol. 2001 Jun;29(3):193-6. doi: 10.1046/j.1442-9071.2001.00402.x.

DOI:10.1046/j.1442-9071.2001.00402.x

PMID:11446467

Abstract

The contribution of rods and cones to the scotopic electroretinogram (ERG) of small animals is unclear, with a recent report suggesting that the mouse has no cone a-wave. The present study considered the contribution of cones to the ERG of the rat. Dark-adapted Long Evans rats (n = 4) had ERG signals collected following a single flash, which stimulated rods and cones (mixed response), or a twin-fash paradigm (short interstimulus interval, 1 s), which isolated cone responses. Rod signals were derived by digital subtraction of the cone signal from the mixed rod/cone ERG. The rat a-wave was found to be dominated by rod responses but cone responses contributed substantially (45%) to post-receptoral waveforms (b-wave) at higher light levels.

摘要

视杆细胞和视锥细胞对小动物暗视视网膜电图（ERG）的贡献尚不清楚，最近有报告表明小鼠没有视锥细胞a波。本研究探讨了视锥细胞对大鼠ERG的贡献。对暗适应的Long Evans大鼠（n = 4），在单次闪光（刺激视杆细胞和视锥细胞，混合反应）或双闪光模式（短刺激间隔，1秒，分离视锥细胞反应）后收集ERG信号。视杆细胞信号通过从混合的视杆/视锥细胞ERG中数字减去视锥细胞信号得出。研究发现，大鼠的a波主要由视杆细胞反应主导，但在较高光强下，视锥细胞反应对视后接收波形（b波）有很大贡献（45%）。