磷酸丙糖异构酶在矮山松（欧洲赤松）中的遗传与亚细胞定位

Inheritance and subcellular localization of triose-phosphate isomerase in dwarf mountain pine (Pinus mugo).

作者信息

Odrzykoski I J

机构信息

Department of Genetics, A. Mickiewicz University, 60-371 Poznan, Miedzychodzka 5, Poland.

出版信息

J Hered. 2001 May-Jun;92(3):271-4. doi: 10.1093/jhered/92.3.271.

DOI:10.1093/jhered/92.3.271

PMID:11447244

Abstract

Several trees with expected heterozygous phenotype for triose-phosphate isomerase (TPI) were discovered in a population of dwarf mountain pine (Pinus mugo Turra) from southern Poland. As the inheritance of this enzyme in pines has not been reported, segregation of allelic variants was tested in eight trees with putative heterozygous phenotypes for two loci, TpiA and TPIB: Linkage between these and some other isozyme loci were studied and evidence for linkage has been found between TpiA and PgdA (r = 0.10) and between TpiB and DiaD (r = 0.36), but in single trees only. The subcellular localization of TPI isozymes was determined by comparing isoenzymes from the total extract with those found in fraction enriched in plastids, prepared by differential gradient centrifugation of cellular organelles. The more slowly migrating TPI-B isozyme is located in plastids.

摘要

在来自波兰南部的矮山松（Pinus mugo Turra）种群中，发现了几棵具有磷酸丙糖异构酶（TPI）预期杂合表型的树。由于尚未报道松树中这种酶的遗传情况，因此在八棵具有两个位点TpiA和TPIB假定杂合表型的树中测试了等位基因变体的分离：研究了这些位点与其他一些同工酶位点之间的连锁关系，并且仅在单棵树中发现了TpiA与PgdA之间（r = 0.10）以及TpiB与DiaD之间（r = 0.36）存在连锁的证据。通过将总提取物中的同工酶与通过细胞器差速梯度离心制备的富含质体的级分中发现的同工酶进行比较，确定了TPI同工酶的亚细胞定位。迁移较慢的TPI-B同工酶位于质体中。