O'Malley D M, Allendorf F W, Blake G M
Biochem Genet. 1979 Apr;17(3-4):233-49. doi: 10.1007/BF00498965.
Techniques are presented to detect 23 isozyme loci in the long-lived perennial plant, ponderosa pine. Meiotically derived megagametophyte from seeds is used to examine directly the segregation of allelic variants. Approximately seven seeds were initially examined for 12 enzymes from each of 47 trees from ten stands throughout the northern Rocky Mountain region. Additional seeds were also examined from selected families to confirm the inheritance of observed electrophoretic variants at 13 polymorphic loci and to estimate linkage relationship. Significant norandom segregation was consistently detected for three pairs of loci: ADH-1:AAT-2, ADH-1:PGI-1, and LAP-2:6PG-1. Preliminary estimates of population parameters reveal a relatively high average heterozygosity (H = 0.123). This is partitioned into a high amont of genetic variation within local stands, with only approximately 12% of the total heterozygosity resulting from genic difference between stands.
本文介绍了检测长寿多年生植物美国黄松中23个同工酶位点的技术。利用种子减数分裂产生的雌配子体直接检测等位基因变体的分离情况。最初从落基山脉北部地区十个林分的47棵树中，选取大约七粒种子检测12种酶。还从选定的家系中检测了更多种子，以确认在13个多态位点观察到的电泳变体的遗传情况，并估计连锁关系。在三对位点上持续检测到显著的非随机分离：ADH-1:AAT-2、ADH-1:PGI-1和LAP-2:6PG-1。群体参数的初步估计显示平均杂合度相对较高（H = 0.123）。这被划分为当地林分内大量的遗传变异，林分间基因差异导致的杂合度仅占总杂合度的约12%。
