Pérez-Gallego L, Moreno-Bueno G, Sarrió D, Suárez A, Gamallo C, Palacios J
Department of Pathology, Hospital del Rio Hortega, Valladolid, Spain.
Am J Clin Pathol. 2001 Jul;116(1):143-8. doi: 10.1309/42U5-Q2EV-RCBM-WPGJ.
We studied 48 human papillomavirus (HPV)-16-positive squamous intraepithelial lesions (SILs) from HIV-negative patients (16 low-grade SILs [LSILs]; 32 high-grade SILs [HSILs]) and 13 HPV-16-positive SILs from HIV-positive patients with AIDS (1 LSIL; 12 HSILs). After HPV typing, the entire HPV-16 E6 coding region was amplified and sequenced in all samples. We detected 12 HPV-16 E6 prototypes and 4 variants among the LSILs in HIV-negative patients, and 15 HPV-16 E6 prototypes and 17 HPV-16 variants in the HSIL group. The most prevalent variant of SIL types was European 350G, present in 3 and 13 cases, respectively. In 3 HSILs and no LSILs we found mixed infection by an HPV-16 E6 prototype and a variant. Two variants (1 each in LSIL and HSIL) were of non-European lineage. The only LSIL in HIV-positive patients had an HPV-16 E6 prototype; in the HSILs, we found 8 HPV-16 E6-prototypes, 4 with mixed infection with HPV-31 and 4 variants, all European 350G. The higher proportion of HPV-16 E6 variants in HSIL than in LSIL in HIV-negative patients suggests a greater risk of progression. However, further studies are needed.
我们研究了48例来自HIV阴性患者的人乳头瘤病毒（HPV）-16阳性鳞状上皮内病变（SIL）（16例低级别SIL [LSIL]；32例高级别SIL [HSIL]）以及13例来自患有艾滋病的HIV阳性患者的HPV-16阳性SIL（1例LSIL；12例HSIL）。进行HPV分型后，对所有样本中的整个HPV-16 E6编码区进行扩增和测序。我们在HIV阴性患者的LSIL中检测到12种HPV-16 E6原型和4种变体，在HSIL组中检测到15种HPV-16 E6原型和17种HPV-16变体。SIL类型中最常见的变体是欧洲350G，分别出现在3例和13例中。在3例HSIL中而非LSIL中，我们发现了HPV-16 E6原型和一种变体的混合感染。两种变体（LSIL和HSIL各1种）属于非欧洲谱系。HIV阳性患者中唯一的LSIL具有HPV-16 E6原型；在HSIL中，我们发现8种HPV-16 E6原型，其中4种与HPV-31混合感染，4种变体均为欧洲350G。在HIV阴性患者中，HSIL中HPV-16 E6变体的比例高于LSIL，这表明进展风险更大。然而，还需要进一步研究。
