用扰动角关联方法探测辐照过的DNA。

Probing irradiated DNA with the perturbed angular correlation method.

作者信息

Tsoulou E, Kalfas C A, Sideris E G

机构信息

Institute of Nuclear Physics, NCSR Demokritos, 153 10 Agia Paraskevi, Athens, Greece.

出版信息

Radiat Res. 2001 Aug;156(2):181-5. doi: 10.1667/0033-7587(2001)156[0181:pidwtp]2.0.co;2.

DOI:10.1667/0033-7587(2001)156[0181:pidwtp]2.0.co;2

PMID:11448239

Abstract

The technique of perturbed angular correlations of gamma rays has been used to study the effects of radiation on DNA molecules. The samples are buffered solutions of calf thymus DNA exposed to various doses (0-80 Gy) of gamma rays. Indium-111 is used as a probe. Rotational correlation times, tau(c), a parameter measuring the flexibility of a macromolecule, are obtained that show a dependence on radiation dose.

摘要

γ射线扰动角关联技术已被用于研究辐射对DNA分子的影响。样品是暴露于不同剂量（0 - 80戈瑞）γ射线的小牛胸腺DNA缓冲溶液。铟 - 111用作探针。获得了旋转相关时间τ(c)，这是一个测量大分子柔韧性的参数，它显示出对辐射剂量的依赖性。