Lee S F, Newton C, Widen R, Friedman H, Klein T W
Department of Medical Microbiology and Immunology, College of Medicine, University of South Florida, 12901 Bruce Downs Boulevard, MDC Box 10, Tampa, FL 33612, USA.
Eur J Pharmacol. 2001 Jul 6;423(2-3):235-41. doi: 10.1016/s0014-2999(01)01122-0.
Cannabinoid CB(2) receptor is reported to be expressed in varying amounts in different human immune subpopulations. To examine the expression pattern of CB(2) in the mouse, immune cell subpopulations were purified and studied by semiquantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR). CB(2) mRNA was most abundant in splenic B cells, followed by macrophages and T cells. Furthermore, CB(2) was expressed in thioglycollate-elicited peritoneal macrophages, but not in resident peritoneal macrophages. In addition to these studies on receptor expression at basal activity, CB(2) mRNA expression was also studied following immune cell activation. Bacterial lipopolysaccharide stimulation downregulated CB(2) mRNA expression in splenocyte cultures in a dose-response manner, while stimulation through cluster of differentiation 40 (CD40) using anti-CD40 antibody upregulated the response and costimulation with interleukin-4 attenuated the anti-CD40 response. These results demonstrate that CB(2) mRNA expression differs among mouse immune subpopulations similar to what is observed in human immune cells. Furthermore, the results suggest that the signaling pathways activated by lipopolysaccharide and anti-CD40 might have different effects on CB(2) mRNA expression.
据报道，大麻素CB(2)受体在不同的人类免疫亚群中表达量各异。为了研究CB(2)在小鼠体内的表达模式，通过半定量逆转录聚合酶链反应（RT-PCR）对免疫细胞亚群进行了纯化和研究。CB(2) mRNA在脾脏B细胞中最为丰富，其次是巨噬细胞和T细胞。此外，CB(2)在巯基乙酸诱导的腹腔巨噬细胞中表达，但在常驻腹腔巨噬细胞中不表达。除了对基础活性下受体表达的这些研究外，还对免疫细胞激活后的CB(2) mRNA表达进行了研究。细菌脂多糖刺激以剂量反应方式下调脾细胞培养物中CB(2) mRNA的表达，而使用抗CD40抗体通过分化簇40（CD40）刺激则上调反应，并且白细胞介素-4共刺激减弱了抗CD40反应。这些结果表明，CB(2) mRNA在小鼠免疫亚群中的表达与在人类免疫细胞中观察到的情况类似。此外，结果表明脂多糖和抗CD40激活的信号通路可能对CB(2) mRNA表达有不同影响。
