Rhim J S, Nelson-Rees W A, Essex M
Int J Cancer. 1979 Sep 15;24(3):336-40. doi: 10.1002/ijc.2910240311.
A feline embryo cell line was treated in vitro with various levels of 7,12-dimethylbenz[a]anthracene (DMBA) or dimethyl sulfoxide (control). Repeat treatment of DMBA only induced in vitro transformation of feline embryo cells following clonal growth and selection. The morphologically altered cells formed large cell aggregates and grew in this aggregate form when suspended in liquid growth medium above an agar base, formed colonies in soft agar, and grew to high saturation densities. However, no progressively growing tumors were produced when cells were inoculated into nude athymic mice. The transformed lines were negative for feline oncornavirus-associated cell membrane antigen (FOCMA).
用不同浓度的7,12-二甲基苯并[a]蒽（DMBA）或二甲基亚砜（对照）对猫胚胎细胞系进行体外处理。仅重复DMBA处理在克隆生长和筛选后诱导猫胚胎细胞发生体外转化。形态改变的细胞形成大的细胞聚集体，当悬浮于琼脂基上方的液体生长培养基中时以这种聚集体形式生长，在软琼脂中形成集落，并生长至高饱和密度。然而，当将细胞接种到无胸腺裸鼠体内时，未产生进行性生长的肿瘤。转化细胞系的猫肿瘤病毒相关细胞膜抗原（FOCMA）呈阴性。
