采用液相色谱法同时测定片剂制剂中可待因和盐酸乙基吗啡的含量。

Simultaneous determination of codeine and ethyl morphine HCL in tablet formulations using LC.

作者信息

Değim T, Akay C, Büyükafşar K, Cevheroğlu S

机构信息

Department of Quality Control, Army Drug Factory, Dişkapi, Ankara, Turkey.

出版信息

J Pharm Biomed Anal. 2001 Aug;26(1):15-21. doi: 10.1016/s0731-7085(01)00392-2.

DOI:10.1016/s0731-7085(01)00392-2

PMID:11451638

Abstract

A reverse phase high performance liquid chromatography (HPLC) method was developed for the simultaneous determination of codeine (methyl morphine) and dionin (ethyl morphine hydrochloride) in antitussive analgesic tablet formulations. A C(18) column and methanol-water (1:2) mixture mobile phase (pH 3.0) were used. Spectrophotometric detection was carried out at 210 nm. The total elution time was shorter than 7 min. This method was found to be quite precise and reproducible. This proposed method was successfully applied to the determination of codeine and ethyl morphine hydrochloride in tablets produced by the Turkish Army Drug Factory.

摘要

建立了一种反相高效液相色谱（HPLC）法，用于同时测定镇咳止痛片剂制剂中的可待因（甲基吗啡）和狄奥宁（盐酸乙基吗啡）。采用C(18)柱和甲醇-水（1:2）混合流动相（pH 3.0）。在210 nm处进行分光光度检测。总洗脱时间短于7分钟。该方法具有很高的精密度和重现性。该方法成功应用于土耳其军队制药厂生产的片剂中可待因和盐酸乙基吗啡的测定。