采用高效液相色谱结合紫外和电化学检测法同时测定尿液中的6-单乙酰吗啡、吗啡和可待因。

Simultaneous determination of 6-monoacetylmorphine, morphine and codeine in urine using high-performance liquid chromatography with combined ultraviolet and electrochemical detection.

作者信息

Gerostamoulos J, Crump K, McIntyre I M, Drummer O H

机构信息

Victorian Institute of Forensic Pathology, Monash University, South Melbourne, Australia.

出版信息

J Chromatogr. 1993 Jul 23;617(1):152-6. doi: 10.1016/0378-4347(93)80435-7.

DOI:10.1016/0378-4347(93)80435-7

PMID:8376530

Abstract

A method is described for the simultaneous determination of 6-monoacetylmorphine (6-MAM), morphine and codeine in post-mortem urine specimens using reversed-phase high-performance liquid chromatography with dual ultraviolet spectrophotometric and electrochemical detection. The limits of detection for a 1-ml urine sample were 0.04 mg/l for 6-MAM and 0.05 mg/l for both morphine and codeine. The presence of 6-MAM in urine indicates prior use of heroin and enables differentiation between morphine- and heroin-related deaths.

摘要

描述了一种使用反相高效液相色谱结合双紫外分光光度法和电化学检测法同时测定死后尿液样本中6-单乙酰吗啡（6-MAM）、吗啡和可待因的方法。对于1毫升尿液样本，6-MAM的检测限为0.04毫克/升，吗啡和可待因的检测限均为0.05毫克/升。尿液中6-MAM的存在表明之前使用过海洛因，并能够区分吗啡相关死亡和海洛因相关死亡。