采用高效液相色谱法同时测定尿液和血液中的可待因和吗啡。

Simultaneous determination of codeine and morphine in urine and blood by HPLC.

作者信息

Posey B L, Kimble S N

出版信息

J Anal Toxicol. 1983 Sep-Oct;7(5):241-5. doi: 10.1093/jat/7.5.241.

DOI:10.1093/jat/7.5.241

Abstract

A procedure is presented for the determination of nanogram quantities of codeine and morphine in biological specimens by HPLC. The quantitative method is based on hydrolysis of the urine samples followed by basic extraction of urine and blood with organic solvents. Separation by liquid chromatography is a reverse phase system on a Spherisorb-CN (5 micron) column with a mobile phase of 40% methanol and phosphate buffer, pH 6.8. The method separated the opiates for screening purposes and was used for confirmation of morphine detected by radioimmunoassay.

摘要

本文介绍了一种通过高效液相色谱法（HPLC）测定生物样本中纳克级可待因和吗啡含量的方法。该定量方法基于尿液样本的水解，然后用有机溶剂对尿液和血液进行碱性萃取。液相色谱分离采用Spherisorb-CN（5微米）柱上的反相系统，流动相为40%甲醇和pH 6.8的磷酸盐缓冲液。该方法用于分离阿片类药物以进行筛查，并用于确证放射免疫分析法检测到的吗啡。