Gonzalez-Billault C, Avila J, Cáceres A
Centro de Biologia Molecular, Consejo Superior de Investigaciones Cientificas, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
Mol Biol Cell. 2001 Jul;12(7):2087-98. doi: 10.1091/mbc.12.7.2087.
Cultured neurons obtained from a hypomorphous MAP1B mutant mouse line display a selective and significant inhibition of axon formation that reflects a delay in axon outgrowth and a reduced rate of elongation. This phenomenon is paralleled by decreased microtubule formation and dynamics, which is dramatic at the distal axonal segment, as well as in growth cones, where the more recently assembled microtubule polymer normally predominates. These neurons also have aberrant growth cone formation and increased actin-based protrusive activity. Taken together, this study provides direct evidence showing that by promoting microtubule dynamics and regulating cytoskeletal organization MAP1B has a crucial role in axon formation.
从一个低表达MAP1B突变小鼠品系获得的培养神经元表现出轴突形成的选择性且显著抑制，这反映了轴突生长延迟和伸长速率降低。这种现象与微管形成和动力学的降低同时出现，在轴突远端段以及生长锥中这种现象很明显，在生长锥中最近组装的微管聚合物通常占主导地位。这些神经元还具有异常的生长锥形成和基于肌动蛋白的突出活动增加。综上所述，本研究提供了直接证据表明，通过促进微管动力学和调节细胞骨架组织，MAP1B在轴突形成中具有关键作用。
