使用电印迹法对纤溶酶原表型进行免疫化学鉴定。

Immunochemical demonstration of plasminogen phenotypes using electroblotting.

作者信息

Pytlik C, Rand S, Brinkmann B

机构信息

Institut für Rechtsmedizin der Universität Münster, Federal Republic of Germany.

出版信息

Int J Legal Med. 1990 Dec;104(1):21-3. doi: 10.1007/BF01816479.

DOI:10.1007/BF01816479

PMID:11453087

Abstract

The polymorphism of plasminogen (PLG) was analysed by isoelectric focusing on polyacrylamide gels, followed by electroblotting. For analysis, neuraminidase-pretreated sera were used. In a random sample of 500 unrelated individuals from east Westphalia 8 phenotypes were observed. The allele frequencies were: PLG1 (A), 0.708; PLG2 (B), 0.274; PLG3 (A3), 0.013; PLGV, 0.005. The family data (300 mother-child pairs) confirmed the hypothesis of autosomal codominant inheritance. PLG phenotypes could be determined in 1-year-old bloodstains (cotton and glass) that had been stored at +4 degrees C and -20 degrees C. The PLG phenotypes could clearly be demonstrated in sera diluted 1:4. The phenotype 1 (A) could still be detected in dilutions of 1:6. The theoretical exclusion rate was calculated to be 22.6%.

摘要

采用聚丙烯酰胺凝胶等电聚焦结合电印迹法分析纤溶酶原（PLG）的多态性。分析时使用了经神经氨酸酶预处理的血清。在来自东威斯特法伦的500名无亲缘关系个体的随机样本中，观察到8种表型。等位基因频率分别为：PLG1（A），0.708；PLG2（B），0.274；PLG3（A3），0.013；PLGV，0.005。家系数据（300对母婴）证实了常染色体共显性遗传的假设。在储存在4℃和-20℃的1年陈旧血迹（棉质和玻璃材质）中能够确定PLG表型。在1:4稀释的血清中可清晰显示PLG表型。在1:6稀释液中仍可检测到表型1（A）。计算得出理论排除率为22.6%。