检测孤立型畸精症患者的 DNA 碎片化和减数分裂分离。
Detection of DNA fragmentation and meiotic segregation in human with isolated teratozoospermia.
机构信息
Department of Cytogenetic and Reproductive biology, Farhat Hached, University Teaching Hospital, Sousse, Tunisia,
出版信息
J Assist Reprod Genet. 2011 Jan;28(1):41-8. doi: 10.1007/s10815-010-9482-8. Epub 2010 Sep 25.
PURPOSE
To evaluate levels of DNA fragmentation and chromosomal abnormalities in ejaculated sperm of males with isolated teratozoospermia and to determine if specific sperm morphological types occur simultaneously with these nuclear defects.
METHODS
Sperm obtained from isolated teratozoospermic men (n = 70) and fertile men (n = 30) were analysed using fluorescence in situ hybridization and TUNEL assay.
RESULTS
Teratozoospermic men, compared to fertile men, showed significantly higher rates of sex chromosomes disomy, and diploidy. Significant correlations were found between amorphous head, microcephalic head, short tail, and sex chromosomes disomy. Level of sperm DNA fragmentation was significantly higher in teratozoospermic men than in controls and positively correlated to the incidence of macrocephalic heads, amorphous heads, and short flagella.
CONCLUSIONS
Patients with isolated teratozoospermia have increased levels of DNA fragmentation and chromosomal aneuploidy. Some specific morphological abnormalities were shown to be predictive of chromosomal abnormalities and DNA alteration.
目的
评估畸形精子症患者射出精子的 DNA 碎片水平和染色体异常情况，并确定是否同时存在这些核缺陷和特定的精子形态类型。
方法
使用荧光原位杂交和 TUNEL 检测分析来自畸形精子症患者（n=70）和正常生育男性（n=30）的精子。
结果
与正常生育男性相比，畸形精子症患者的性染色体非整倍体和二倍体率显著更高。不规则头、小头、短尾与性染色体非整倍体之间存在显著相关性。畸形精子症患者的精子 DNA 碎片化水平明显高于对照组，且与大头、不规则头和短鞭毛的发生率呈正相关。
结论
孤立性畸形精子症患者的 DNA 碎片化和染色体非整倍体水平增加。一些特定的形态学异常被证明可预测染色体异常和 DNA 改变。
Zotero 插件