Suppr超能文献

末端反转录转座子激活果蝇2L端粒处的亚端粒白色转基因。

Terminal retrotransposons activate a subtelomeric white transgene at the 2L telomere in Drosophila.

作者信息

Golubovsky M D, Konev A Y, Walter M F, Biessmann H, Mason J M

机构信息

Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709-2233, USA.

出版信息

Genetics. 2001 Jul;158(3):1111-23. doi: 10.1093/genetics/158.3.1111.

Abstract

Genetically marked P elements inserted into the subtelomeric satellites of Drosophila show repression and variegation of the reporter gene. One such white+ reporter, inserted between the subtelomeric satellite and the terminal HeT-A array in the left arm of chromosome 2 (2L), is sensitive to its context; changes in the structure of the telomere region can be identified by changes in eye color. Addition of HeT-A or TART elements to the 2L terminus increases w+ expression, and loss of sequence from the end decreases expression. This indicates that the telomeric retrotransposons in Drosophila have an activating influence on the repressed subterminal reporter gene. Changes in eye color due to altered expression of the transgene also allow the detection of interactions between homologous telomeres. The 2L arms that terminate in long HeT-A/TART arrays showed increased expression of the subterminal w+ transgene when the terminal repeats on the homologue are absent or markedly shorter. We propose that the chromatin structure of the terminal HeT-A/TART array and the activity of a putative promoter/enhancer element on HeT-A are affected by telomeric interactions. Such trans-activation may reflect control over HeT-A transcription and, thus, transposition activity.

摘要

插入果蝇亚端粒卫星区域的基因标记P元件会导致报告基因的抑制和斑驳现象。其中一个这样的白色+报告基因插入在2号染色体左臂(2L)的亚端粒卫星和末端HeT - A阵列之间,对其所处环境敏感;端粒区域结构的变化可通过眼睛颜色的变化来识别。向2L末端添加HeT - A或TART元件会增加w +的表达,而末端序列的缺失则会降低表达。这表明果蝇中的端粒逆转座子对被抑制的亚末端报告基因具有激活作用。由于转基因表达改变导致的眼睛颜色变化也能检测到同源端粒之间的相互作用。当同源染色体上的末端重复序列缺失或明显缩短时,以长HeT - A/TART阵列结尾的2L臂显示出亚末端w +转基因的表达增加。我们推测,末端HeT - A/TART阵列的染色质结构以及HeT - A上一个假定的启动子/增强子元件的活性受端粒相互作用的影响。这种反式激活可能反映了对HeT - A转录的控制,进而反映了转座活性。

相似文献

1
Terminal retrotransposons activate a subtelomeric white transgene at the 2L telomere in Drosophila.
Genetics. 2001 Jul;158(3):1111-23. doi: 10.1093/genetics/158.3.1111.
4
Directional gene silencing induced by a complex subtelomeric satellite from Drosophila.
Chromosoma. 1998 Nov;107(5):311-20. doi: 10.1007/s004120050313.
5
Control of telomere elongation and telomeric silencing in Drosophila melanogaster.
Genetica. 2000;109(1-2):61-70. doi: 10.1023/a:1026548503320.
6
7
Coevolution of the telomeric retrotransposons across Drosophila species.
Genetics. 2002 Jul;161(3):1113-24. doi: 10.1093/genetics/161.3.1113.
8
Two distinct domains in Drosophila melanogaster telomeres.
Genetics. 2005 Dec;171(4):1767-77. doi: 10.1534/genetics.105.048827. Epub 2005 Sep 2.
9
HeT-A elements in Drosophila virilis: retrotransposon telomeres are conserved across the Drosophila genus.
Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):14091-6. doi: 10.1073/pnas.1936193100. Epub 2003 Nov 12.
10
Silence at the End: How Drosophila Regulates Expression and Transposition of Telomeric Retroelements.
J Mol Biol. 2020 Jul 10;432(15):4305-4321. doi: 10.1016/j.jmb.2020.06.004. Epub 2020 Jun 5.

引用本文的文献

1
Effete, an E2 ubiquitin-conjugating enzyme with multiple roles in Drosophila development and chromatin organization.
Fly (Austin). 2013 Oct-Dec;7(4):256-62. doi: 10.4161/fly.26567. Epub 2013 Oct 2.
3
Protein landscape at Drosophila melanogaster telomere-associated sequence repeats.
Mol Cell Biol. 2012 Jun;32(12):2170-82. doi: 10.1128/MCB.00010-12. Epub 2012 Apr 9.
4
Telomere-targeted retrotransposons in the rice blast fungus Magnaporthe oryzae: agents of telomere instability.
Genetics. 2012 Jun;191(2):389-406. doi: 10.1534/genetics.111.137950. Epub 2012 Mar 23.
5
Retrotransposons at Drosophila telomeres: host domestication of a selfish element for the maintenance of genome integrity.
Biochim Biophys Acta. 2012 Jul;1819(7):771-5. doi: 10.1016/j.bbagrm.2012.01.018. Epub 2012 Feb 4.
6
Differential maintenance of DNA sequences in telomeric and centromeric heterochromatin.
Genetics. 2011 Jan;187(1):51-60. doi: 10.1534/genetics.110.122994. Epub 2010 Nov 1.
7
Regulation of telomere length in Drosophila.
Cytogenet Genome Res. 2008;122(3-4):356-64. doi: 10.1159/000167823. Epub 2009 Jan 30.
9
HP1 is distributed within distinct chromatin domains at Drosophila telomeres.
Genetics. 2008 Sep;180(1):121-31. doi: 10.1534/genetics.108.090647. Epub 2008 Aug 24.
10
Drosophila telomeres: an exception providing new insights.
Bioessays. 2008 Jan;30(1):25-37. doi: 10.1002/bies.20688.

本文引用的文献

1
Functional analysis of the white gene of Drosophila by P-factor-mediated transformation.
EMBO J. 1984 Sep;3(9):2077-85. doi: 10.1002/j.1460-2075.1984.tb02094.x.
2
Control of telomere elongation and telomeric silencing in Drosophila melanogaster.
Genetica. 2000;109(1-2):61-70. doi: 10.1023/a:1026548503320.
3
P-Element repression in Drosophila melanogaster by a naturally occurring defective telomeric P copy.
Genetics. 2000 Aug;155(4):1841-54. doi: 10.1093/genetics/155.4.1841.
5
Transvection and other homology effects.
Curr Opin Genet Dev. 1999 Apr;9(2):237-46. doi: 10.1016/S0959-437X(99)80035-5.
6
Directional gene silencing induced by a complex subtelomeric satellite from Drosophila.
Chromosoma. 1998 Nov;107(5):311-20. doi: 10.1007/s004120050313.
7
Mechanisms of silencing in Saccharomyces cerevisiae.
Curr Opin Genet Dev. 1998 Apr;8(2):233-9. doi: 10.1016/s0959-437x(98)80146-9.
8
Silent chromatin in yeast: an orchestrated medley featuring Sir3p [corrected].
Bioessays. 1998 Jan;20(1):30-40. doi: 10.1002/(SICI)1521-1878(199801)20:1<30::AID-BIES6>3.0.CO;2-W.
9
Chromosome ends: all the same under their caps.
Curr Opin Genet Dev. 1997 Dec;7(6):822-8. doi: 10.1016/s0959-437x(97)80046-9.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验