Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
EMBO J. 1984 Sep;3(9):2077-85. doi: 10.1002/j.1460-2075.1984.tb02094.x.
A 12-kb DNA segment spanning the white (w) locus of Drosophila has been inserted into a P-transposon vector and used for P-factor-mediated germ-line transformation. Several red-eyed transformants were recovered which complement the white mutant phenotype. Analysis of the eye pigments and the interaction with the zeste mutation indicates that the w gene inserted at several new chromosomal sites is expressed normally. The tissue-specific accumulation of w transcripts, as studied by in situ hybridization to tissue sections, is the same in transformant and wild-type larvae. This indicates that all the genetic information specified by the w locus is contained within this 12-kb segment of DNA. By secondary mobilization it was shown that the w sequences have been inserted as a functional P(w) transposon which is capable of further transposition.
一段跨越果蝇白色（w）基因座的 12kb DNA 片段已被插入 P 转座子载体中，并用于 P 因子介导的生殖系转化。已经回收了几个红眼转化体，它们弥补了白色突变表型。对眼睛色素的分析和与 zeste 突变的相互作用表明，插入几个新染色体位置的 w 基因正常表达。通过原位杂交组织切片研究 w 转录本的组织特异性积累，在转化体和野生型幼虫中是相同的。这表明 w 基因座所指定的所有遗传信息都包含在这个 12kb 的 DNA 片段中。通过二次动员，表明 w 序列已作为一个功能 P(w)转座子插入，该转座子能够进一步转座。
