通过比较基因组杂交技术检测宫颈癌9号染色体短臂的扩增情况。
Amplification at 9p in cervical carcinoma by comparative genomic hybridization.
作者信息
Jee K J, Kim Y T, Kim K R, Aalto Y, Knuutila S
机构信息
Department of Medical Genetics, Haartman Institute and Helsinki University Central Hospital, University of Helsinki, Finland.
出版信息
Anal Cell Pathol. 2001;22(3):159-63. doi: 10.1155/2001/174645.
DNA copy number changes were studied by comparative genomic hybridization on 10 tumor specimens of squamous cell carcinoma of cervix obtained from Korean patients. DNA was extracted from paraffin-embedded sections after removal of non-malignant cells by microdissection technique. Copy number changes were found in 8/10 tumors. The most frequent changes were chromosome 19 gains (n=6) and losses on chromosomes 4 (n=4), 5 (n=3), and 3p (n=3). A novel finding was amplification in chromosome arm 9p21-pter in 2 cases. Gains in 1, 3q, 5p, 6p, 8q, 16p, 17, and 20q and losses at 2q, 6q, 8p, 9q, 10p, 11, 13, 16q, and 18q were observed in at least one of the cases.
采用比较基因组杂交技术对10例来自韩国患者的宫颈鳞状细胞癌肿瘤标本进行DNA拷贝数变化研究。通过显微切割技术去除非恶性细胞后，从石蜡包埋切片中提取DNA。在10个肿瘤中有8个发现了拷贝数变化。最常见的变化是19号染色体增加（n = 6）以及4号（n = 4）、5号（n = 3）和3p（n = 3）染色体缺失。一个新发现是2例患者9p21 - pter染色体臂出现扩增。至少在1例患者中观察到1号、3q、5p、6p、8q、16p、17号和20q染色体增加以及2q、6q、8p、9q、10p、11、13、16q和18q染色体缺失。
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