Kuroda N, Sato D, Ohyama K, Wada M, Nakahara Y, Nakashima K
School of Pharmaceutical Sciences, Nagasaki University, Japan.
Chem Pharm Bull (Tokyo). 2001 Jul;49(7):905-8. doi: 10.1248/cpb.49.905.
Separation of twelve sympathomimetic amines and related compounds by micellar electrokinetic chromatography (MEKC) with UV absorbance detection is described. These amines were well separated within 25 min using 50 mM sodium tetraborate solution containing 15 mM sodium dodecylsulfate (SDS) of pH 9.3 as a running solution and detected at 210 nm. MEKC was performed with an applied voltage of 13 kV at 25 degrees C using a fused-silica capillary (50 cm x 75 mm i.d.) with effective length of 37.5 cm. The detection limits of these compounds were in the range from 4 to 97 fmol/injection at a signal-to-noise ratio (S/N) of 3. The reproducibility of the method expressed as relative standard deviation (RSD) for within-day (n=6) and between-day (n=5) assays was less than 4.8 and 8.8%, respectively. The proposed method could be applied to the determination of an anorectic drug, phentermine, in Chinese tea with a detection limit of 99 microg/g (105 fmol/injection, S/N=3).
描述了采用胶束电动色谱法（MEKC）结合紫外吸收检测对十二种拟交感神经胺及相关化合物进行分离的方法。使用pH 9.3的含15 mM十二烷基硫酸钠（SDS）的50 mM硼酸钠溶液作为运行溶液，这些胺类在25分钟内得到了良好分离，并在210 nm处进行检测。在25℃下，使用有效长度为37.5 cm的熔融石英毛细管（50 cm×75 mm内径），以13 kV的施加电压进行MEKC。这些化合物在信噪比（S/N）为3时的检测限在4至97 fmol/进样范围内。该方法日内（n = 6）和日间（n = 5）测定的重现性分别以相对标准偏差（RSD）表示，均小于4.8%和8.8%。该方法可应用于测定中国茶中的食欲抑制剂苯丁胺，检测限为99 μg/g（105 fmol/进样，S/N = 3）。
