Zelter Alex, Bencina Mojca, Bowman Barry J, Yarden Oded, Read Nick D
Fungal Cell Biology Group, Institute of Cell Biology, University of Edinburgh, UK.
Fungal Genet Biol. 2004 Sep;41(9):827-41. doi: 10.1016/j.fgb.2004.05.001.
A large number of Ca2+ -signaling proteins have been previously identified and characterized in Saccharomyces cerevisiae but relatively few have been discovered in filamentous fungi. In this study, a detailed, comparative genomic analysis of Ca2+ -signaling proteins in Neurospora crassa, Magnaporthe grisea, and S. cerevisiae has been made. Our BLAST analysis identified 48, 42, and 40 Ca2+ -signaling proteins in N. crassa, M. grisea, and S. cerevisiae, respectively. In N. crassa, M. grisea, and S. cerevisiae, 79, 100, and 13% of these proteins, respectively, were previously unknown. For N. crassa, M. grisea, and S. cerevisiae, respectively, we have identified: three Ca2+ -permeable channels in each species; 9, 12, and 5 Ca2+/cation-ATPases; eight, six, and four Ca2+ -exchangers; four, four, and two phospholipase C's; one calmodulin in each species; and 23, 21, and 29 Ca2+/calmodulin-regulated proteins. Homologs of a number of key proteins involved in the release of Ca2+ from intracellular stores, and in the sensing of extracellular Ca2+, in animal and plant cells, were not identified. The greater complexity of the Ca2+ -signaling machinery in N. crassa and M. grisea over that in S. cerevisiae probably reflects their more complex cellular organization and behavior, and the greater range of external signals which filamentous fungi have to respond to in their natural habitats. To complement the data presented in this paper, a comprehensive web-based database resource (http://www.fungalcell.org/fdf/) of all Ca2+ -signaling proteins identified in N. crassa, M. grisea, and S. cerevisiae has been provided.
此前已在酿酒酵母中鉴定并表征了大量的Ca2+信号蛋白，但在丝状真菌中发现的相对较少。在本研究中，对粗糙脉孢菌、稻瘟病菌和酿酒酵母中的Ca2+信号蛋白进行了详细的比较基因组分析。我们的BLAST分析分别在粗糙脉孢菌、稻瘟病菌和酿酒酵母中鉴定出48、42和40种Ca2+信号蛋白。在粗糙脉孢菌、稻瘟病菌和酿酒酵母中，这些蛋白分别有79%、100%和13%此前未知。对于粗糙脉孢菌、稻瘟病菌和酿酒酵母，我们分别鉴定出：每个物种中有三种Ca2+通透通道；9种、12种和5种Ca2+/阳离子ATP酶；8种、6种和4种Ca2+交换器；4种、4种和2种磷脂酶C；每个物种中有1种钙调蛋白；以及23种、21种和29种Ca2+/钙调蛋白调节蛋白。未鉴定出动物和植物细胞中参与从细胞内储存库释放Ca2+以及感知细胞外Ca2+的许多关键蛋白的同源物。粗糙脉孢菌和稻瘟病菌中Ca2+信号机制比酿酒酵母中的更复杂，这可能反映了它们更复杂的细胞组织和行为，以及丝状真菌在其自然栖息地中必须响应的更广泛的外部信号。为补充本文提供的数据，已提供了一个基于网络的综合数据库资源（http://www.fungalcell.org/fdf/），其中包含在粗糙脉孢菌、稻瘟病菌和酿酒酵母中鉴定出的所有Ca2+信号蛋白。
