Zamecnik J, Barbe G, Moger W H, Armstrong D T
Steroids. 1977 Nov;30(5):679-89. doi: 10.1016/0039-128x(77)90057-5.
Androsterone (3alpha-hydroxy-5alpha-androstan-17-one), 5alpha-androstane-3alpha, 17beta-diol and 5alpha-androstane-3beta, 17beta-diol were conjugated at C-16 through sulfur to bovine and human serum albumin. Rabbits injected with these conjugates produced antibodies suitable for radioimmunoassays of these hormone metabolites. Samples were purified on Sephadex LH-20 columns. Levels of these steroids were measured in a rat blood serum pool and in ovarian tissue extract pools.
雄酮（3α-羟基-5α-雄甾烷-17-酮）、5α-雄甾烷-3α,17β-二醇和5α-雄甾烷-3β,17β-二醇通过硫在C-16位与牛血清白蛋白和人血清白蛋白结合。注射这些结合物的兔子产生了适用于这些激素代谢物放射免疫分析的抗体。样品在葡聚糖凝胶LH-20柱上进行纯化。在大鼠血清池和卵巢组织提取物池中测量了这些类固醇的水平。
