Cremer F, Lönnig W E, Saedler H, Huijser P
Max-Planck Institut für Züchtungsforschung, Carl-von-Linné Weg 10, 50829 Köln, Germany.
Plant Physiol. 2001 Jul;126(3):1031-41. doi: 10.1104/pp.126.3.1031.
The snapdragon (Antirrhinum majus) centroradialis mutant (cen) is characterized by the development of a terminal flower, thereby replacing the normally open inflorescence by a closed inflorescence. In contrast to its Arabidopsis counterpart, terminal flower1, the cen-null mutant displays an almost constant number of lateral flowers below the terminal flower. Some partial revertants of an X-radiation-induced cen mutant showed a delayed formation of the terminal flower, resulting in a variable number of lateral flowers. The number of lateral flowers formed was shown to be environmentally controlled, with the fewer flowers formed under the stronger flower-inducing conditions. Plants displaying this "Delayed terminal flower" phenotype were found to be heterozygous for a mutant allele carrying a transposon in the coding region and an allele from which the transposon excised, leaving behind a 3-bp duplication as footprint. As a consequence, an iso-leucine is inserted between Asp148 and Gly149 in the CENTRORADIALIS protein. It is proposed that this mutation results in a low level of functional CEN activity, generating a phenotype that is more similar to the Arabidopsis Terminal flower phenotype.
金鱼草(Antirrhinum majus)辐射中心突变体(cen)的特征是形成顶花,从而用闭合的花序取代正常开放的花序。与其拟南芥对应物终端花1不同,cen基因缺失突变体在顶花下方的侧花数量几乎恒定。X射线诱导的cen突变体的一些部分回复突变体显示顶花形成延迟,导致侧花数量可变。已证明形成的侧花数量受环境控制,在较强的成花诱导条件下形成的花较少。发现表现出这种“延迟顶花”表型的植物对于在编码区携带转座子的突变等位基因和转座子从中切除的等位基因是杂合的,留下一个3碱基对的重复作为足迹。结果,在辐射中心蛋白的Asp148和Gly149之间插入了一个异亮氨酸。有人提出,这种突变导致功能性CEN活性水平较低,产生一种与拟南芥终端花表型更相似的表型。