Maier J, Hecker R, Rockel P, Ninnemann H
Institute for Plant Biochemistry, Eberhard-Karls-University, Corrensstrasse 41, D-72076 Tübingen, Germany.
Plant Physiol. 2001 Jul;126(3):1323-30. doi: 10.1104/pp.126.3.1323.
Blue light controls the development of sporangiophores in the zygomycete Phycomyces blakesleeanus Burgeff. Light represses the production of microsporangiophores and enhances the development of macrosporangiophores. Inhibition of the biosynthesis of tetrahydrobiopterin, a cofactor of NO synthase, inhibits this photomorphogenesis. Light induces production of citrulline from arginine in the mycelium and in sporangiophores. The citrulline-forming activity is dependent on NADPH, independent of calcium, and inhibited by NO synthase inhibitors. It is reduced in tetrahydrobiopterin-depleted mycelium. Light induces emission of NO from the developing fungus in the same order of magnitude as citrulline formation from arginine. The NO donor sodium nitroprusside can replace the light effect on sporangiophore development, and inhibitors of NO synthase repress it. We suggest that a fungal NO synthase is involved in sporangiophore development and propose its participation in light signaling.
蓝光控制接合菌黑根霉（Phycomyces blakesleeanus Burgeff）中孢子囊梗的发育。光抑制小孢子囊梗的产生并促进大孢子囊梗的发育。抑制一氧化氮合酶的辅因子四氢生物蝶呤的生物合成会抑制这种光形态建成。光诱导菌丝体和孢子囊梗中精氨酸生成瓜氨酸。瓜氨酸形成活性依赖于NADPH，不依赖于钙，并被一氧化氮合酶抑制剂抑制。在四氢生物蝶呤缺乏的菌丝体中其活性降低。光诱导发育中的真菌释放一氧化氮，其数量与精氨酸形成瓜氨酸的数量处于同一量级。一氧化氮供体硝普钠可以替代光对孢子囊梗发育的影响，而一氧化氮合酶抑制剂则会抑制这种影响。我们认为真菌一氧化氮合酶参与孢子囊梗的发育，并提出其参与光信号传导。
