高良姜果实及其伪品的nrDNA ITS区序列特征

Characters of nrDNA ITS region sequences of fruits of Alpinia galanga and their adulterants.

作者信息

Zhao Z L, Zhou K Y, Dong H, Xu L S

出版信息

Planta Med. 2001 Jun;67(4):381-3. doi: 10.1055/s-2001-14311.

DOI:10.1055/s-2001-14311

Abstract

The fruits of Alpinia galanga (L.) Sw. are used as a traditional Chinese medicine; but the dry fruits of A. conchigera, A. suishaensis, A. maclurei and A. polyantha are also used as the medicine in local areas. Because dry fruits of these related plants are similar to those of Alpinia galanga (L.) Sw. in odor, morphological characters and chemical components, and even anatomical characters, it is difficult to identify the medicine. Nuclear ribosomal DNA internal transcribed spacer (ITS) regions of the five taxa were directly sequenced using an automated sequencer. Sequence analysis showed that the ITS 1 ranges from 177 to 178 base pairs (bp), and the ITS 2 from 225 to 234 bp. The size of the 5.8S coding region is 164 bp for all species. Also, the pairwise sequence divergence is higher and some molecular markers were determined. According to these molecular markers, Alpinia galanga (L.) Sw. and the related species can easily be distinguished from each other. Therefore, evidence from nrDNA ITS sequence variation can identify the medicine at the DNA level.

摘要

大高良姜的果实被用作传统中药；但在当地，红壳砂仁、缩砂、海南假砂仁及多花砂仁的干燥果实也被用作药物。由于这些近缘植物的干燥果实在气味、形态特征、化学成分甚至解剖特征方面都与大高良姜的果实相似，因此很难对这些药物进行鉴别。使用自动测序仪对这五个分类群的核糖体DNA内转录间隔区（ITS）区域进行直接测序。序列分析表明，ITS 1的长度为177至178个碱基对（bp），ITS 2的长度为225至234 bp。所有物种的5.8S编码区大小均为164 bp。此外，两两序列差异较高，并确定了一些分子标记。根据这些分子标记，可以很容易地将大高良姜与相关物种区分开来。因此，nrDNA ITS序列变异的证据可以在DNA水平上对这些药物进行鉴别。