Wallace H W, Liquori E M, Stein T P, Brooks H
Trans Am Soc Artif Intern Organs. 1975;21:450-4.
Thrombocytopenia and platelet dysfunction are well known complications of extracorporeal circulation. The production of ""denatured'' protein is a common occurrence with extracorporeal circulation even in the absence of a blood-gas interface. We subjected platelet-poor plasma to vigorous bubbling with air and studied the effect of this ""denatured'' plasma on platelets from the same donor. Aggregation induced by ADP, epinephrine or collagen was measured. The bubbled plasma decreased the delay in the onset of aggregation observed with collagen and eliminated the biphasic aggregation response induced by epinephrine. These data indicate that ""denatured'' protein may affect platelet function and may play a role in the thrombocytopenia and altered platelet function associated with extracorporeal circulation.
血小板减少症和血小板功能障碍是体外循环众所周知的并发症。即使在没有血气界面的情况下，“变性”蛋白质的产生在体外循环中也很常见。我们让少血小板血浆与空气剧烈鼓泡，并研究这种“变性”血浆对来自同一供体的血小板的影响。测量了由二磷酸腺苷（ADP）、肾上腺素或胶原蛋白诱导的聚集情况。鼓泡后的血浆减少了胶原蛋白诱导的聚集起始延迟，并消除了肾上腺素诱导的双相聚集反应。这些数据表明，“变性”蛋白质可能会影响血小板功能，并可能在与体外循环相关的血小板减少症和血小板功能改变中起作用。
