Livingstone D R, Mitchelmore C L, Peters L D, O'Hara S C, Shaw J P, Chesman B S, Doyotte A, McEvoy J, Ronisz D, Larsson D G, Förlin L
Centre for Coastal and Marine Sciences, Plymouth Marine Laboratory, Citadel Hill, Plymouth, Devon PL1 2PB, UK.
Mar Environ Res. 2000 Jul-Dec;50(1-5):367-71. doi: 10.1016/s0141-1136(00)00060-x.
The potential of eel (Anguilla anguilla) as a monitoring species for the Thames Estuary, UK, was examined. Hepatic cytochrome P4501A [7-ethoxyresorufin O-deethylase (EROD) activity] and blood vitellogenin (Western analysis) were investigated as biomarkers of exposure to, respectively, organic contaminants and to contaminants showing estrogenic activity. Hepatic microsomal EROD activities in A. anguilla from seven sites in the Thames Estuary in May 1998 varied three-fold (111 +/- 24 to 355 +/- 42 pmol min-1 mg protein-1) (mean +/- S.E.M.) and showed correlation with salinity; however, the latter relationship was not maintained at other times of the year. The range of EROD activities was two- to eight-fold higher than the 37 +/- 8 pmol min-1 mg-1 for A. anguilla from the relatively clean Tamar Estuary. beta-Naphthoflavone treatment (5 mg kg-1 wet wt.; 2 days) of Thames A. anguilla produced a two-fold increase in hepatic microsomal EROD activity. Comparing the Thames EROD data with those for A. anguilla from well-characterised contaminated sites in the Netherlands (Van der Oost, R., Goksøyr, A., Celander, M., Heida, H., & Vermeulen, N. P. E. 1996. Aquatic Toxicology, 36, 189-222), the Thames is suggested to be moderately impacted by polycyclic aromatic hydrocarbons and related contaminants. 17-beta-Estradiol treatment produced the appearance of a plasma protein of 211 Kd app. mol. wt. (recognised by antibodies to vitellogenin of Morone saxatilis), but putative vitellogenin could not be detected in A. anguilla from selected sites in the Thames Estuary.
对欧洲鳗鲡（Anguilla anguilla）作为英国泰晤士河口监测物种的潜力进行了研究。分别将肝脏细胞色素P4501A[7-乙氧基异吩恶唑酮O-脱乙基酶（EROD）活性]和血液卵黄蛋白原（蛋白质免疫印迹分析）作为有机污染物暴露和具有雌激素活性污染物暴露的生物标志物进行研究。1998年5月，取自泰晤士河口7个地点的欧洲鳗鲡肝脏微粒体EROD活性变化了3倍（111±24至355±42 pmol·min⁻¹·mg蛋白质⁻¹）（平均值±标准误），且与盐度相关；然而，在一年中的其他时间这种关系并不成立。与取自相对清洁的塔马河口的欧洲鳗鲡（其EROD活性为37±8 pmol·min⁻¹·mg⁻¹）相比，泰晤士河口欧洲鳗鲡的EROD活性范围高出2至8倍。用β-萘黄酮（5 mg·kg⁻¹湿重；处理2天）处理泰晤士河口的欧洲鳗鲡，其肝脏微粒体EROD活性增加了两倍。将泰晤士河口欧洲鳗鲡的EROD数据与取自荷兰特征明确的污染地点的欧洲鳗鲡数据（Van der Oost, R., Goksøyr, A., Celander, M., Heida, H., & Vermeulen, N. P. E. 1996. Aquatic Toxicology, 36, 189 - 222）进行比较，表明泰晤士河口受到多环芳烃及相关污染物的中度影响。用17-β-雌二醇处理后，出现了一种表观分子量为211 Kd的血浆蛋白（可被条纹鲈卵黄蛋白原抗体识别），但在泰晤士河口选定地点的欧洲鳗鲡中未检测到假定的卵黄蛋白原。
