Pacheco M, Santos M A
Department of Biology, University of Aveiro, Portugal.
Ecotoxicol Environ Saf. 1998 May-Jun;40(1-2):71-6. doi: 10.1006/eesa.1998.1644.
The induction of liver ethoxyresorufin O-deethylase (EROD) activity and erythrocytic nuclear abnormalities (NA) after treatment with two different polycyclic aromatic hydrocarbons (PAHs) and cyclophosphamide (CP) was investigated in the eel Anguilla anguilla L. EROD activity significantly increases either 3 days after one single i.p. injection or 6 days after two i.p. injections (on days 0 and 3) of 4 mg/kg beta-naphthoflavone (BNF). EROD activity was determined after different lengths of exposure to 4 mg/kg BNF (i.p. injection). The results indicated significant increases from 8-h to 12-day exposure, with a maximum increase at 4 days and a decline between 4 and 12 days. The induction of liver EROD activity and erythrocytic NA was studied, 3 days after one 14.7-mumol/kg treatment (i.p. injection) with BNF, benzo[a]pyrene (BaP), and CP, in three three different groups of eels. The EROD activity significantly increases after BNF and BaP treatment, whereas the erythrocytic NA frequency was kept constant; however, CP induces a significant increase in the erythrocytic NA frequency, but it does not induce a significant increase in liver EROD activity. The sensitivity of A. anguilla liver EROD assay was assessed 3 days after one i.p. injection of BNF (0.0, 0.03, 0.06, 0.125, 0.25, 0.5, 1.0, 2.0, and 4.0 mg/kg). This species exhibits a dose response to BNF at the concentration range 0-4 mg/kg. The NOEL is between 0.125 and 0.25 mg/kg. The eels 3-day exposure to water containing BNF (0.0, 0.1, 0.3, 0.9, and 2.7 microM) induced a significant increase in liver EROD activity, specifically in concentrations of 0.9 and 2.7 microM. The NOEC for the eels external exposure to BNF is between 0.3 and 0.9 microM.
在欧洲鳗鲡（Anguilla anguilla L.）中研究了用两种不同的多环芳烃（PAHs）和环磷酰胺（CP）处理后肝脏乙氧基异吩恶唑酮-O-脱乙基酶（EROD）活性和红细胞核异常（NA）的诱导情况。单次腹腔注射4 mg/kgβ-萘黄酮（BNF）3天后或两次腹腔注射（第0天和第3天）后6天，EROD活性显著增加。在不同时长暴露于4 mg/kg BNF（腹腔注射）后测定EROD活性。结果表明，从8小时到12天的暴露过程中EROD活性显著增加，在第4天达到最大增加，在4至12天之间下降。在三组不同的欧洲鳗鲡中，研究了用14.7 μmol/kg BNF、苯并[a]芘（BaP）和CP进行一次腹腔注射处理3天后肝脏EROD活性和红细胞NA的诱导情况。BNF和BaP处理后EROD活性显著增加，而红细胞NA频率保持不变；然而，CP诱导红细胞NA频率显著增加，但不诱导肝脏EROD活性显著增加。在单次腹腔注射BNF（0.0、0.03、0.06、0.125、0.25、0.5、1.0、2.0和4.0 mg/kg）3天后评估欧洲鳗鲡肝脏EROD测定的敏感性。该物种在0 - 4 mg/kg的浓度范围内对BNF表现出剂量反应。无观察到有害作用水平（NOEL）在0.125至0.25 mg/kg之间。欧洲鳗鲡暴露于含有BNF（0.0、0.1、0.3、0.9和2.7 μM）的水中3天诱导肝脏EROD活性显著增加，特别是在0.9和2.7 μM的浓度下。欧洲鳗鲡外部暴露于BNF的无观察到效应浓度（NOEC）在0.3至0.9 μM之间。
