枯草芽孢杆菌孢子在孢子萌发中间阶段受阻时的特性。
Properties of spores of Bacillus subtilis blocked at an intermediate stage in spore germination.
作者信息
Setlow B, Melly E, Setlow P
机构信息
Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA.
出版信息
J Bacteriol. 2001 Aug;183(16):4894-9. doi: 10.1128/JB.183.16.4894-4899.2001.
Abstract
Germination of mutant spores of Bacillus subtilis unable to degrade their cortex is accompanied by excretion of dipicolinic acid and uptake of some core water. However, compared to wild-type germinated spores in which the cortex has been degraded, the germinated mutant spores accumulated less core water, exhibited greatly reduced enzyme activity in the spore core, synthesized neither ATP nor reduced pyridine or flavin nucleotides, and had significantly higher resistance to heat and UV irradiation. We propose that the germinated spores in which the cortex has not been degraded represent an intermediate stage in spore germination, which we term stage I.
摘要
无法降解其皮层的枯草芽孢杆菌突变体孢子的萌发伴随着吡啶二羧酸的排泄和一些核心水的摄取。然而，与皮层已被降解的野生型萌发孢子相比，萌发的突变体孢子积累的核心水较少，孢子核心中的酶活性大大降低，既不合成ATP，也不合成还原型吡啶或黄素核苷酸，并且对热和紫外线辐射具有明显更高的抗性。我们提出，皮层未被降解的萌发孢子代表孢子萌发的一个中间阶段，我们将其称为I期。
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