Chen P, Eggleston P A
Johns Hopkins University, 600 North Wolfe Street, Baltimore, MD 21287, USA.
Clin Exp Allergy. 2001 Jul;31(7):1086-93. doi: 10.1046/j.1365-2222.2001.01127.x.
To facilitate allergen removal from indoor environments, it would be helpful to have household cleaning products that modified allergenic activity. Because NaOCl dissolves proteins in high concentrations and is both capable of killing bacteria and viruses and inactivating viral antigens at somewhat lower concentrations, we explored its effects on Mus m 1 and other indoor allergens.
To examine the ability of NaOCl to reduce the allergenicity of Mus m 1 and other indoor allergens.
Using purified mouse urinary allergen, we examined the effect on protein measured by Coomassie protein assay and on Mus m 1 measured by ELISA. We also examined the effects using SDS/PAGE and Western blots probed with sheep anti-Mus m 1 and with allergic human serum.
When NaOCl and Mus m 1 were combined in a molar ratio of 100 : 1, IgE binding to Mus m 1 on Western blot was significantly reduced. At higher NaOCl concentrations the protein appeared to fragment and eventually became undetectable. Fragmentation appeared to be random in that peptides of a wide range of apparent molecular weight were produced. The reaction was complete within 1-2 min at OCl : pr ratios of greater than 200 : 1 and was optimal at pH 7.4. Immunological activity of other allergens (Fel d 1, Bla g 1, Der p 1) was decreased in vitro and dried allergen extracts were removed from surfaces. Adding an extraneous protein, BSA, to NaOCl:Mus m 1 solutions decreased the effect of NaOCl on the allergen.
We concluded that NaOCl at concentrations commonly used in household products is capable of dramatically affecting allergenic protein.
为便于从室内环境中去除过敏原，拥有能改变过敏原活性的家用清洁产品将很有帮助。由于高浓度的次氯酸钠（NaOCl）能溶解蛋白质，并且既能杀死细菌和病毒，又能在稍低浓度下使病毒抗原失活，我们探究了其对小鼠Mus m 1及其他室内过敏原的影响。
检测次氯酸钠降低Mus m 1及其他室内过敏原致敏性的能力。
我们使用纯化的小鼠尿液过敏原，通过考马斯亮蓝蛋白测定法检测其对蛋白质的影响，并通过酶联免疫吸附测定法检测对Mus m 1的影响。我们还使用十二烷基硫酸钠/聚丙烯酰胺凝胶电泳（SDS/PAGE）以及用羊抗Mus m 1和过敏人血清进行免疫印迹检测其影响。
当次氯酸钠与Mus m 1以100∶1的摩尔比混合时，免疫印迹上与Mus m 1结合的免疫球蛋白E（IgE）显著减少。在更高的次氯酸钠浓度下，蛋白质似乎发生了片段化，最终变得无法检测到。片段化似乎是随机的，因为产生了一系列表观分子量范围很广的肽段。在OCl∶pr比例大于200∶1时，反应在1 - 2分钟内完成，且在pH 7.4时最为理想。其他过敏原（猫毛蛋白（Fel d 1）、黑曲霉1（Bla g 1）、屋尘螨1（Der p 1））的免疫活性在体外降低，并且干燥的过敏原提取物从表面被去除。向次氯酸钠∶Mus m 1溶液中添加额外的蛋白质牛血清白蛋白（BSA）会降低次氯酸钠对过敏原的作用。
我们得出结论，家用产品中常用浓度的次氯酸钠能够显著影响致敏蛋白。
