Lityńska A, Przybyło M, Pocheć E, Hoja-Łukowicz D, Ciołczyk D, Laidler P, Gil D
Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Ingardena 6, 30-060 Kraków, Poland.
Melanoma Res. 2001 Jun;11(3):205-12. doi: 10.1097/00008390-200106000-00001.
Glycosylation is generally altered in tumour cells in comparison with their normal counterparts. These alterations are thought to be important because they contribute to the abnormal behaviour of cancer cells. Therefore, we have comparatively analysed the glycoproteins in cell extracts from human melanoma (primary site--WM35; metastatic sites-- WM239, WM9 and A375) cell lines using sodium dodecyl sulphate-polyacrylamide gel electrophoresis and lectin staining. The glycoprotein pattern of the WM35 line differed from that of the other cell lines in having less proteins that reacted with Sambucus nigra, Maackia amurensis and Phaseolus vulgaris agglutinins. A glycoprotein of about 70 kDa had a significantly increased reaction with Sambucus nigra agglutinin in all the cell lines from metastatic sites. In the WM9, WM239 and A375 cell lines, additional bands (160-100 kDa) were stained with Phaseolus vulgaris agglutinin, suggesting that cells from metastatic sites contain more glycoproteins with beta1-6 branches. On the other hand, only minor changes in the reaction with Galanthus nivalis agglutinin, a mannose-specific lectin, were detected. Among the proteins showing different lectin staining, one, with an apparent molecular weight of 133 kDa, was recognized by antibodies as N-cadherin. The present results suggest that in human melanoma the expression of branched and sialylated complex type N-oligosaccharides consistently increased in cells from metastatic sites, and support the view that carbohydrates are associated with the acquisition of the metastatic potential of tumour cells.
与正常细胞相比，肿瘤细胞中的糖基化通常会发生改变。这些改变被认为很重要，因为它们导致癌细胞的异常行为。因此，我们使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和凝集素染色，对来自人黑色素瘤（原发部位——WM35；转移部位——WM239、WM9和A375）细胞系的细胞提取物中的糖蛋白进行了比较分析。WM35细胞系的糖蛋白模式与其他细胞系不同，与黑接骨木、山槐和菜豆凝集素反应的蛋白质较少。在所有来自转移部位的细胞系中，一种约70 kDa的糖蛋白与黑接骨木凝集素的反应显著增加。在WM9、WM239和A375细胞系中，菜豆凝集素染色显示出额外的条带（160 - 100 kDa），表明来自转移部位的细胞含有更多带有β1-6分支的糖蛋白。另一方面，与甘露糖特异性凝集素雪花莲凝集素的反应仅检测到微小变化。在显示不同凝集素染色的蛋白质中，有一种表观分子量为133 kDa的蛋白质被抗体识别为N-钙黏蛋白。目前的结果表明，在人黑色素瘤中，来自转移部位的细胞中分支和唾液酸化的复合型N-寡糖的表达持续增加，并支持碳水化合物与肿瘤细胞转移潜能获得相关的观点。
