Benallal M, Zotter H, Anner R M, Lacotte D, Moosmayer M, Anner B M
Laboratory of Experimental Cell Therapeutics, Geneva University Medical School, Switzerland.
Biochem Biophys Res Commun. 1995 Apr 26;209(3):921-9. doi: 10.1006/bbrc.1995.1586.
Altered glycosylations of cell surface glycoproteins often accompany malignant transformation and lectins are useful for probing these alterations. Lymphocytes exhibit characteristic surface glycoproteins which serve as markers of cell status and development. The present work was undertaken to compare, on blots, the binding characteristics of membranes isolated from normal peripheral blood lymphocytes and chronic lymphatic leukemia cells to five different lectins, from Datura stramonium, Maackia amurensis, Sambucus nigra, Galanthus nivalis and Peanut. The Maackia amurensis lectin interacted with the normal lymphocytes but showed no binding to malignant cells. Hence, we suggest the Maackia lectin may be used to differentiate normal from leukemic cells.
细胞表面糖蛋白糖基化的改变常伴随恶性转化，凝集素有助于探究这些改变。淋巴细胞表现出特征性的表面糖蛋白，这些糖蛋白可作为细胞状态和发育的标志物。本研究旨在通过印迹法比较从正常外周血淋巴细胞和慢性淋巴细胞白血病细胞分离的膜与五种不同凝集素（来自曼陀罗、黑水缬草、黑接骨木、雪花莲和花生）的结合特性。黑水缬草凝集素与正常淋巴细胞相互作用，但与恶性细胞无结合。因此，我们认为黑水缬草凝集素可用于区分正常细胞和白血病细胞。
