Viñas Salas J, Piñol Felis C, Fermiñan A, Egido R, Pérez-Holanda S, Biendicho P, Buenestado J, Reñé Espinet J
Departments of Medicine and Surgery, Lleida University School of Medicine, Arnau de Vilanova University Hospital, Lleida, Spain.
Rev Esp Enferm Dig. 2001 Mar;93(3):140-7.
To investigate the effect of repetitive mucosal trauma, anastomosis and intestinal content on experimental colonic carcinogenesis as there is the possibility than non-specific colon lesions can promote cancer.
We performed to sixty female Sprague-Dawley rats a 4 cm colon loop defunctionalization with double colostomy (traumatic site). Intestinal continuity was restored with an end-to-end colo-colic silk anastomosis. The surviving 47 rats were divided in 3 groups: Group A: 27 rats treated with DMH. Group B: 10 rats treated with EDTA and Group C: Control of 10 rats. Animals were sacrificed 31-32 weeks after surgery for macro and micropathological studies.
In group A appeared 60 tumours: 44 in the functional colon, 20 of them in the anastomotic site; 8 in the non traumatised defunctionalized segment and 18 in the traumatised segment (p < 0.05).
a) Continuous microtraumas on colonic mucosa in rats are cancer promotional factors; b) silk suture in anastomosis promotes cancer.
鉴于非特异性结肠病变可能促进癌症发生，研究重复性黏膜创伤、吻合术及肠内容物对实验性结肠癌发生的影响。
我们对60只雌性斯普拉格-道利大鼠进行了4厘米结肠袢去功能化并做双结肠造口术（创伤部位）。通过端端结肠-结肠丝线吻合术恢复肠道连续性。存活的47只大鼠分为3组：A组：27只大鼠用二甲基肼处理。B组：10只大鼠用乙二胺四乙酸处理，C组：作为对照的10只大鼠。术后31 - 32周处死动物进行大体和显微病理研究。
A组出现60个肿瘤：44个在功能性结肠，其中20个在吻合部位；8个在未受创伤的去功能化节段，18个在受创伤节段（p < 0.05）。
a）大鼠结肠黏膜的持续性微创伤是癌症促进因素；b）吻合术中的丝线缝合促进癌症发生。
