Hubens G, Lafullarde T, Van Marck E, Vermeulen P, Hubens A
Department of Surgery, Academic Hospital University, Antwerp, Belgium.
Acta Chir Belg. 1994 Sep-Oct;94(5):258-62.
The ability of malignant colon cancer cells to implant on intact and damaged colonic mucosa and serosa was tested in a series of experiments using male WAG rats. The mucosa was damaged in an "acute" way by multiple biopsies and in a "chronic" way by means of a chemically induced colitis. The creation of an anastomosis provoked both mucosal and serosal damages. CC531 colon cancer cells injected intraluminally as an enema implanted at the serosal side of an anastomosis without invading the mucosa in 66% of the rats. An intact colon mucosa is 100% resistant to the implantation of viable CC531 cells. In contrast, tumour growth was noticed in 100% of the rats when the malignant cells were sprayed over the intact colon serosa at laparotomy. Mucosal damage by biopsies in the presence of viable colon cancer cells resulted in mucosal implantation and intraluminal tumour growth in one out of thirty rats. No tumour growth was observed when the CC531 cells were instilled on one week old mucosal ulcerations resulting from a chemical induced colitis.
在一系列实验中，使用雄性WAG大鼠测试了恶性结肠癌细胞在完整和受损结肠黏膜及浆膜上着床的能力。通过多次活检以“急性”方式损伤黏膜，通过化学诱导结肠炎以“慢性”方式损伤黏膜。吻合术的创建引发了黏膜和浆膜损伤。作为灌肠剂经腔内注射的CC531结肠癌细胞，在66%的大鼠中植入吻合口的浆膜侧，而未侵入黏膜。完整的结肠黏膜对存活的CC531细胞植入具有100%的抵抗力。相比之下，当在剖腹手术时将恶性细胞喷洒在完整的结肠浆膜上时，100%的大鼠出现肿瘤生长。在存在存活结肠癌细胞的情况下，活检造成的黏膜损伤导致30只大鼠中有1只出现黏膜植入和腔内肿瘤生长。当将CC531细胞滴注在化学诱导结肠炎导致的一周龄黏膜溃疡上时，未观察到肿瘤生长。
