Badylak S, Kokini K, Tullius B, Whitson B
Department of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
J Surg Res. 2001 Aug;99(2):282-7. doi: 10.1006/jsre.2001.6176.
The change in strength over time of a biomaterial derived from the small intestinal submucosa (SIS) was determined in a dog model of body wall repair. Full-thickness body wall defects measuring 8 x 12 cm were surgically created and then repaired with a multilaminate eight-layer form of SIS in 40 dogs. Five dogs were sacrificed at each of the following time points: 1 day, 4 days, 7 days, 10 days, and 1, 3, 6, and 24 months. Ball burst tests that measured biaxial ultimate load-bearing capability were performed on the device prior to implantation and on the device/implant site at the time of sacrifice. The strength of the device at the time of implant was approximately 73 +/- 12 pounds. The strength of the implant site diminished to 40 +/- 18 pounds at 10 days, and then progressively increased to a value of 156 +/- 26 pounds at 24 months (P < 0.05). The clinical utility of a degradable biomaterial such as SIS depends on a balance between the rate of degradation and the rate of host remodeling. Naturally occurring extracellular matrix scaffolds such as SIS show rapid degradation with associated and subsequent remodeling to a tissue with strength that exceeds that of the native tissue when used as a body wall repair device.
在体壁修复的犬模型中，测定了源自小肠黏膜下层（SIS）的生物材料随时间的强度变化。通过手术制造了40只犬的全层体壁缺损，缺损尺寸为8×12厘米，然后用多层八层形式的SIS进行修复。在以下每个时间点处死5只犬：1天、4天、7天、10天、1个月、3个月、6个月和24个月。在植入前对该装置以及在处死时对装置/植入部位进行了测量双轴极限承载能力的球囊爆破试验。植入时该装置的强度约为73±12磅。植入部位的强度在10天时降至40±18磅，然后逐渐增加，在24个月时达到156±26磅（P<0.05）。诸如SIS之类的可降解生物材料的临床效用取决于降解速率与宿主重塑速率之间的平衡。当用作体壁修复装置时，诸如SIS之类的天然存在的细胞外基质支架显示出快速降解，并伴随着随后重塑为一种强度超过天然组织的组织。
