Badylak S F, Tullius R, Kokini K, Shelbourne K D, Klootwyk T, Voytik S L, Kraine M R, Simmons C
Hillenbrand Biomedical Engineering Center, Purdue University, West Lafayette, Indiana 47907, USA.
J Biomed Mater Res. 1995 Aug;29(8):977-85. doi: 10.1002/jbm.820290809.
A study was conducted to evaluate the tissue response to a xenogeneic biomaterial when this material was used to repair an experimentally induced Achilles tendon defect in the dog. Twenty dogs had a 1.5 cm segmental defect of the Achilles tendon created surgically which was then repaired with acellular connective tissue derived from porcine small intestinal submucosa (SIS). The animals were sacrificed at 1, 2, 4, 8, 12, 16, 24, and 48 weeks and the neotendons examined for uniaxial longitudinal tensile strength, morphologic appearance, hydroxyproline (collagen) content, and disappearance of the originally implanted SIS material over time. The contralateral normal Achilles tendons served as controls as did four additional dogs that had a 1.5 cm segmental Achilles tendon defect created surgically without subsequent surgical repair with SIS. Results showed the SIS remodeled neotendons to be stronger than the musculotendinous origin or the boney insertion (> 1000 N) by 12 weeks after surgery and to consist of organized collagen-rich connective tissue similar to the contralateral normal tendons. The four dogs in which no SIS was implanted showed inferior strength at the comparable time points of 4, 8, 12, and 16 weeks. Immunohistochemical studies suggest that the SIS biomaterial becomes degraded within the first eight weeks and serves as a temporary scaffold around which the body deposits appropriate and organized connective tissue. SIS is a promising biomaterial worthy of further investigation for orthopedic soft tissue applications.
进行了一项研究，以评估一种异种生物材料在用于修复犬实验性诱导的跟腱缺损时的组织反应。20只犬通过手术制造了1.5厘米的跟腱节段性缺损，然后用源自猪小肠黏膜下层（SIS）的脱细胞结缔组织进行修复。在1、2、4、8、12、16、24和48周时处死动物，检查新生肌腱的单轴纵向拉伸强度、形态外观、羟脯氨酸（胶原蛋白）含量以及随着时间推移最初植入的SIS材料的消失情况。对侧正常跟腱作为对照，另外4只通过手术制造了1.5厘米跟腱节段性缺损但未用SIS进行后续手术修复的犬也作为对照。结果显示，SIS重塑的新生肌腱在术后12周时比肌肉肌腱起点或骨附着点更强（>1000 N），并且由与对侧正常肌腱相似的有组织的富含胶原蛋白的结缔组织组成。在4、8、12和16周的可比时间点，未植入SIS的4只犬的强度较低。免疫组织化学研究表明，SIS生物材料在最初八周内会降解，并作为一个临时支架，身体在其周围沉积适当且有组织的结缔组织。SIS是一种有前景的生物材料，值得在骨科软组织应用方面进行进一步研究。
