胰岛素改变不均一核核糖核蛋白K与DNA和RNA的蛋白结合。
Insulin alters heterogeneous nuclear ribonucleoprotein K protein binding to DNA and RNA.
作者信息
Ostrowski J, Kawata Y, Schullery D S, Denisenko O N, Higaki Y, Abrass C K, Bomsztyk K
机构信息
Department of Medicine, University of Washington, Seattle, WA 98195, USA.
出版信息
Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9044-9. doi: 10.1073/pnas.161284098. Epub 2001 Jul 24.
Abstract
The interaction of the multimodular heterogeneous nuclear ribonucleoprotein (hnRNP) K protein with many of its protein and nucleic acid partners is regulated by extracellular signals. Acting as a docking platform, K protein could link signal-transduction pathways to DNA- and RNA-directed processes such as transcription, mRNA processing, transport, and translation. Treatment of hepatocyte culture with insulin increased K protein tyrosine phosphorylation. Insulin altered K protein interaction with RNA and DNA in vitro. Administration of insulin into mice had similar effects on K protein in liver. Coimmunoprecipitations of RNA with K protein revealed preferential in vivo K protein binding of a subset of transcripts, including the insulin-inducible c-fos mRNA. These results suggest a class of insulin pathways that signal nucleic acid-directed processes that involve K protein.
摘要
多模块异质性核糖核蛋白（hnRNP）K蛋白与其众多蛋白质和核酸伴侣之间的相互作用受细胞外信号调控。作为一个对接平台，K蛋白可将信号转导通路与DNA和RNA导向的过程（如转录、mRNA加工、转运和翻译）联系起来。用胰岛素处理肝细胞培养物可增加K蛋白酪氨酸磷酸化。胰岛素在体外改变了K蛋白与RNA和DNA的相互作用。给小鼠注射胰岛素对肝脏中的K蛋白有类似影响。K蛋白与RNA的共免疫沉淀显示，在体内K蛋白优先结合一部分转录本，包括胰岛素诱导的c-fos mRNA。这些结果提示了一类涉及K蛋白的胰岛素信号通路，该通路可对核酸导向的过程发出信号。
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