Iyer R, Liveris D, Adams A, Nowakowski J, McKenna D, Bittker S, Cooper D, Wormser G P, Schwartz I
Department of Biochemistry & Molecular Biology, New York Medical College, Valhalla, New York 10595, USA.
J Clin Microbiol. 2001 Aug;39(8):2954-7. doi: 10.1128/JCM.39.8.2954-2957.2001.
Genetic diversity among Borrelia burgdorferi isolates recovered from the skin of Lyme disease patients was assessed by ribosomal DNA (rDNA) spacer restriction fragment length polymorphism analysis, genomic restriction site polymorphism analysis, and plasmid content analysis. There was a significant association between the three rDNA spacer types, the six pulsed-field gel types, and plasmid content (P < 0.001). The association between distinct chromosomal and plasmid markers implies a clonal origin for each genotype.
通过核糖体DNA(rDNA)间隔区限制性片段长度多态性分析、基因组限制性酶切位点多态性分析和质粒含量分析,对从莱姆病患者皮肤中分离出的伯氏疏螺旋体菌株之间的遗传多样性进行了评估。三种rDNA间隔区类型、六种脉冲场凝胶类型和质粒含量之间存在显著关联(P < 0.001)。不同的染色体和质粒标记之间的关联意味着每种基因型都有克隆起源。